Software Module MALDI Biotyper Changing Microbiology nnovation with Integri

Selective Testing for Beta-Lactamase Activity Antibiotics are often the only effective treatment for severe illnesses caused by microorganisms. Therefore, bacterial antibiotic resistance poses a significant threat to public health worldwide. An important resistance mechanism is enzymatic drug inactivation by extended-spectrum beta-lactamases (ESBLs) and carbapenemases. These bacterial enzymes deactivate β-lactam antibiotics by hydrolyzing the antibiotics’ β-lactam ring. The MBT Compass STAR-BL software module enables fast and accurate analysis of the level of β-lactamase activity in bacterial...

A Fast and Simple Workflow To determine the level of p-lactamase activity in a bacterial culture, samples from overnight cultures or positive blood cultures are incubated in an antibiotic solution. After incubation and centrifugation, cell-free supernatant is spotted onto a MALDI target plate and overlaid with matrix. Spectra are then acquired using the MALDI Biotyper. In cultures where no p-lactamase activity is present, peaks corresponding to the intact antibiotic will be present in the mass spectrum. In cultures where P-lactamase activity is present, peaks corresponding to hydrolyzation products...

Efficient Calibration for Accurate Analysis MALDI-TOF mass spectra signals in the mass range between 100 and 1000 Da. MALDI-TOF mass spectrum of MBT STAR antibiotic calibration standard (MBT STAR-ACS) For research use only. Not for use in diagnostic procedures. Bruker Daltonik GmbH Bremen · Germany Phone +49 (0)421-2205-0 Fax +49 (0)421-2205-103 [email protected] Billerica, MA · USA Phone +1 (978) 663-3660 Fax +1 (978) 667-5993 [email protected] Fremont, CA · USA Phone +1 (510) 683-4300 Fax +1 (510) 687-1217 [email protected] to change specifications without notice. © BDAL 08-2014, 1831507 Calibrating...