Opterra Multipoint Scanning Confocal Microscope
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Catalog excerpts

Opterra Multipoint Scanning Confocal Microscope - 1

Opterra Multipoint Scanning Confocal Microscope Cell-Friendly, High-Speed, High-Resolution Imaging Innovation with Integrity Fluorescence Microscopy

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Opterra Multipoint Scanning Confocal Microscope - 2

Multipoint Scanning Confocal Microscope Superior Integration, Versatility, and Cell-Friendly Performance The Opterra™ Multipoint Scanning Confocal Microscope is designed specifically for live-cell microscopy. It uniquely combines the resolution of traditional confocal systems with the speed typically associated with wide-field imaging. The system utilizes a CCD camera as a detector, and can be configured through selectable aperture sizes so the optimal balance of speed and resolution for each experiment can be set to meet your specific application requirements. Opterra’s unconventional...

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Opterra Multipoint Scanning Confocal Microscope - 3

Resolution and Speed Matched to Applications Efficient and Effective Fluorescence Confocal One aspect of Opterra’s unique scanner design is a motorized aperture plate that contains pinholes of three different sizes and slits of four different widths. Pinholes provide maximum resolution, while slits allow for higher speed acquisitions. This enables the scientist to match aperture size to the objective and optimize the system for specific applications. Plus, aperture selection is controlled by software, so hardware changes are not required for setting up different experiment protocols....

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Opterra Multipoint Scanning Confocal Microscope - 4

Easy, Versatile, High-Performance Data Acquisition Opterra tightly integrates scanner, CCD camera, and motion control devices to provide high-speed, timed volumetric, 4D imaging. Piezo Z-focus can be combined with stage movement for efficient collection of 3D stage montages, as well as timed acquisition at multiple stage locations in individual sample chambers or multi-well plates. Stage montage of zebrafish. Opterra is powered by Prairie View, the same software that drives Bruker’s multiphoton microscope systems. Prairie View provides scientists with a user-friendly interface that allows...

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Opterra Multipoint Scanning Confocal Microscope - 5

Flexible and Feature-Rich Software Multifield Imaging Simplified Prairie View’s Atlas Imaging module makes setting up stage montages and other types of multiple stage location paradigms simple and intuitive. Easy navigation in X, Y and Z allows the user to quickly find and record individual locations of interest, or define a grid of overlapping locations which can be used to construct a high-resolution image of up to an entire specimen. The preview window shows a coarse tiling of all positions scanned, and the live window shows the currently selected field of view. From the quick-scan...

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Opterra Multipoint Scanning Confocal Microscope - 6

Image + Activate = Discover Simultaneous Confocal Imaging and Photoactivation Opterra’s unique optical design allows photostimulation to occur simultaneously with imaging with no loss of image emission signal. The optional photoactivation module uses the same powerful scanning technology found in Bruker’s multiphoton systems. It can be interfaced with UV and visible lasers, as well as with pulsed IR lasers. Opterra provides a comprehensive solution for FRAP ablation, photo-damage, , photoconversion, optogenetics, and uncaging. Photoconversion of Dendra in glioma cell using masked area....

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Opterra Multipoint Scanning Confocal Microscope - 7

Dynamic Spectral Scanning for Live Cell Imaging Opterra’s Spectral Scanning feature performs spectral imaging at speeds appropriate for live cell and small organism imaging. By incorporating an Amici prism into the Opterra image scanner, full spectral data sets of 15 channels can be acquired at speeds of up to four spectral data sets per second for a 512x512 pixel image. Prairie View software provides real time processing of spectral data sets to provide a live image in which up to four channels of data can be visualized. Spectral Scanning also can be used with time lapse, Z-series, and...

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Opterra Multipoint Scanning Confocal Microscope - 8

Scanhead Scanning Method Combination galvanometer and piezoelectric crystal scanning Fully motorized aperture plate with 7 software-selectable apertures; 3 pinhole settings (30, 45, and 60 µm); 4 slit settings (22, 35, 50, and 70 µm) Scan Speeds Scanner or camera master timed imaging; Up to 50 fps in pinhole mode; Up to 1000 fps in slit imaging mode (with appropriate camera) Motorized 6-postion emission filter wheel; Custom dichroics and polychroics for multiple excitation/emission wavelengths Frame trigger out and frame trigger in; 4 auxillary device triggers Image Collection Camera port...

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