The Ultima Family of Multiphoton Microscopes
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Catalog excerpts

The Ultima Family of Multiphoton Microscopes - 1

Ultima Multiphoton Microscope Systems Leading Advances in Life Science Discovery with Fast, Deep Multiphoton Microscopy Innovation with Integrity Fluorescence Microscopy

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The Ultima Family of Multiphoton Microscopes - 2

Ultima Multiphoton Microscope Systems At the forefront of scientific discoveries for more than ten years, Ultima Multiphoton Microscope Systems are the direct result of decades of laboratory experience and close collaboration with leading biological scientists around the world. The involvement of these life science experts has led to a host of unique capabilities and features found only on Ultima systems, such as their modular setup, incorporated photostimulation light path, and Prairie View software. Only Ultima delivers the combination of flexibility, resolution, imaging depth and speed,...

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The Ultima Family of Multiphoton Microscopes - 3

A Legendary Design Keeps Getting Better From the introduction of the first commercial system to allow simultaneous imaging and uncaging in 2003, to the latest intravital system with its resonant scanner and simultaneous optogenetic stimulation, Ultima multiphoton microscopes have grown into the systems of choice for cutting-edge research around the world. Robust and Reliable Technology Ultima systems provide reliable operation in high-use environments from the moment installation is complete because our manufacturing and installation processes are based on more than ten decades of combined...

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The Ultima Family of Multiphoton Microscopes - 4

Ultima IntraVital Designed specifically for intravital studies, this system provides the imaging depth and resolution required for the most demanding live-animal research applications. The Ultima IntraVital is the result of a continuous innovation process, and offers the following advances: An upright light path with extended clearance under the objective Precise triggering for integration with external behavioral trial and electrophysiology systems High-speed volumetric imaging options Optogenetics options A moving X,Y platform option for simplified positioning of the objective with...

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The Ultima Family of Multiphoton Microscopes - 5

Ultima In Vitro The Ultima In Vitro Multiphoton Microscope System is the platform on which leading scientists have conducted breakthrough experiments in brain-slice studies related to neural signaling and neural network mapping. Ultima In Vitro provides simultaneous imaging and electrophysiology, plus uncaging and photostimulation for the complete stimulus and recording environment required for research by today’s neuroscientist. Available on upright or inverted optical microscopes Precise triggering for integration with electrophysiology systems High-speed volumetric imaging options...

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The Ultima Family of Multiphoton Microscopes - 6

Unmatched Options for Extensibility The true power of the Ultima systems lies in their ability to be custom configured. An array of options is available to extend the systems to meet your particular research needs: The Photostimulation Path provides the capability for simultaneous photostimulation or uncaging with imaging. It works with fast-pulsed IR, UV, or visible laser inputs, and accommodates multiple scan types. It also will precisely synchronize with imaging and external triggers. The Orbital Nosepiece provides off-axis imaging (objective tilt and rotation) to access specimen areas...

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The Ultima Family of Multiphoton Microscopes - 7

Superior Options for High-Speed Imaging Current and future research requires high-speed, full-field, and volumetric imaging to meet the demands and growth of emerging application areas. Bruker’s Ultima offers options for increased speed, providing an unmatched ability to capture fast cellular dynamics, moving multiphoton imaging to the next level. Resonant scanner imaging of neuronal signaling, indicated by changes in calcium level of layer 2/3 cells in somatosensory cortex of an awake mouse during behavioral trial. Changes in calcium level measured using GCaMP . The Resonant Scanner...

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The Ultima Family of Multiphoton Microscopes - 8

Ultima Specifications Scanning Method Matched pair of 3 mm Cambridge galvonometers; upgradable to 6 mm Scan Size Pre-set images sizes from 64 x 64 to 2048 x 2048 60x objective: ~200 µm x 200 µm; 40x objective: ~300 µm x 300 µm; 20x objective: ~600 µm x 600 µm Scan Speeds at 1x Zoom Raster scan: 1.65 fps at 512 x 512, >12 fps at 64 x 64; Spiral scan: 6 fps at 512 x 512, >30 fps at 64 x 64 Scan Customization User-definable straight, freehand, and circular (infinite) linescan with included software; user-definable pixels per line and lines per scan from 1 to 2048; up to 128x scan zoom; 360° of...

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