TWINLED Light Source Compact and optimised LED illuminator Fast, Stable Calcium Imaging We introduced LED based Fura-2 systems five years ago, but only with 360nm isosbestic ratioing (due to limitations in LED availability). We are delighted to now offer full 340nm / 380nm ratiometric Fura-2 imaging with integrated excitation filters. Similarly, the LED configuration for Fura-8 is also available. Ratiometric calcium imaging with LEDs can work very well, but, due to the relatively narrow bandwidths and spectral variations of individual LEDs, it does require attention to detail. The FuraLED head works directly with our OptoLED controller and makes the process simple, as we take care of the LED selection and intensity regulation to make your experiments work. We understand that most researchers will also want to use visible indicators and, quite likely, their existing light source alongside the FuraLED. To simplify this the coupling is equipped with a straight-through port to accommodate any fibre based illuminator, or additional Cairn LEDs. APPLICATIONS Fast calcium imaging and photometry FuraLED / GFP coupling combination KEY BENEFITS Full range of microscope / macroscope couplings available Sub-millisecond digital and analogue switching Compatible with existing OptoLED controllers and Cairn microscope couplings OptoLED controller provides dual digital inputs for combined software and direct camera connection to reduce photobleaching Couple to Cairn Multistream for calcium imaging at maximum camera frame rates email: [email protected] [email protected]

ILLUMINATION SYSTEMS INTENSITY, STABILITY AND FLEXIBILITY MultiLine LaserBank Modular and versatile laser launch system allows for use of up to six solid-state lasers from multiple manufacturers. Ideal for TIRF, spinning disk confocal, FRAP and optogenetic applications or any combination of these with multiple outlets via single or multi-mode fibres. Provides the convenience of a custom, turnkey system. TriLine Laser Bank The TriLine shares much of the modularity and flexibility of the MultiLine, but in a simpler and more compact package (up to 3 lasers). The design offers the flexibility to...