1. Segre, G.V., Niall H.D., Habener J.F. et. al. : Metabolism of parathyroid hormone: physiological and clinical significance. Am. J. Med. 56: 774,1974. 2. Mallete, L.E., Gagel, R.F.: Parathyroid Hormone and Calcitonin. In: Murray J.F. (ed) Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism. American Society for Bone and Mineral Research, Kelseyville; William Byrd Press, Richmond, pp. 65-69, 1990. 3. Bilezikian, J.P.: Primary Hyperparathyroidism. In: Murray J.F. (ed) Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism. American Society for Bone and Mineral Research, Kelseyville; William Byrd Press, Richmond, pp. 109-111, 1990. 4. Stewart, A.F.: Humoral Hypercalcemia of Malignancy. In: Murray J.F. (ed) Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism. American Society for Bone and Mineral Research, Kelseyville; William Byrd Press, Richmond, pp. 115-118, 1990. 5. Mallette, L.E.: The parathyroid polyhormones: New concepts in the spectrum of peptide hormone action. Endocrin. Rev. 12:110-117, 1991. 6. Kruger, L.., Rosenblum, S., Zaazra, J. and Wong, J. Intact PTH is stable in unfrozen EDTA plasma for 48 hours prior to laboratory Analysis. Clin. Chem. 41:6: page S47, 1995. 7. Nakamoto, Jon M., Mason, Patrick W. Endocrinology: Test Selection and Interpretation. PTH, Intact and Calcium. Page 123. 2012. 8. Tietz: Clinical Guide to Laboratory Tests, 4th ed, pp. 822-825, 2006 2017-08-22 CALBIOTECH A life science company Bio-intact PTH [Parathyroid Hormone] ELISA Catalog No. PT360T (96 Tests) INTENDED USE The Calbiotech Inc. Bio-intact PTH (BiPTH) ELISA Kit is intended for the quantitative determination of 1-84aa PTH (Parathyroid Hormone) in human serum or plasma. The kit has zero cross reaction to 7-84aa PTH fragment. For Research Use Only. Not for use in diagnostic procedures. PRINCIPLE OF THE TEST The Calbiotech Bio-intact PTH (BiPTH) immunoassay is an adapted two-site sandwich ELISA. In this assay, standards and patient samples are simultaneously incubated with the enzyme labeled detection antibody (specific to 39-84 aa residue) and a biotin coupled capture antibody (specific to 1-6 aa residue) in a streptavidin-coated microplate well. At the end of the assay incubation, the microwell is washed to remove unbound components and the enzyme bound to the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stopping solution is then added to stop the reaction and converts the color to yellow. The intensity of the yellow color is directly proportional to the concentration of intact PTH in the sample. Standards are used to generate a dose response curve of absorbance unit vs. concentration. Concentrations of intact PTH present in the controls and patient samples are determined directly from this curve. Cat #: PT360T (96 Tests) For Order and Inquiries, please contact Mil Calbiotech Inc., 11935 Cordell Ct„ El Cajon, CA 92020 Tel (619) 660-6162 | Fax (619) 660-6970 | Web www.calbiotech.com 1. Distilled or deionized water 3. Disposable pipette tips 4. ELISA reader capable of reading absorbance at 450nm 5. Absorbance paper or paper towel 6. Graph paper STORAGE AND STABILITY 2. Keep microwells sealed in a dry bag with desiccants. 3. The reagents are stable until expiration of the kit. 4. Do not expose reagents to heat, sun, or strong light.

Example of a Standard Curve 1. Potential biohazardous materials: The calibrators contain human source components which have been tested and found non-reactive for hepatitis B surface antigen as well as HIV antibody with FDA licensed reagents. However, as there is no test method that can offer complete assurance that HIV, Hepatitis B virus or other infectious agents are absent, these reagents should be handled at the Biosafety Level 2, as recommended in the Centers for Disease Control/National Institutes of Health manual, "Biosafety in Microbiological and Biomedical Laboratories." 1984 2. This...