1. Catalogs
  2. Calbiotech
  3. Mouse/Rat Cotinine ELISA

Mouse/Rat Cotinine ELISA

Mouse/Rat Cotinine ELISA

Mouse/Rat Cotinine ELISA

Product catalog summary
Intended Use: The Mouse/Rat Cotinine ELISA kit is designed for research purposes only and is not suitable for diagnostic procedures.
Materials Provided: The kit includes a microwell coated with polyclonal antibodies to Cotinine, a standard set, Cotinine HRP enzyme conjugate, TMB substrate, stop solution, and a 20X wash concentrate.
Materials Not Provided: Users need to supply distilled or deionized water, precision pipettes, disposable pipette tips, an ELISA reader capable of reading absorbance at 450nm, absorbance paper, and graph paper.
Storage and Stability: Store the kit at 2-8°C. Keep microwells sealed with desiccants. Reagents are stable until the kit's expiration date. Avoid exposing reagents to heat, sun, or strong light.
Warnings and Precautions: The kit is for laboratory use only and may contain biohazardous materials. Handle reagents at Biosafety Level 2. Do not pipette by mouth or consume food and drinks in the lab area. Use components as an integral unit and do not mix different lots. Run standards, controls, and samples in duplicate for optimal results.
Specimen Handling: The kit is suitable for Mouse/Rat urine or serum. Sodium azide in specimens can affect the assay.
Reagent Preparation: Prepare 1X wash buffer by diluting the 20X concentrate with distilled or deionized water.
Assay Procedure: Bring all reagents to room temperature before use. Pipette standards, controls, and specimens into wells, add enzyme conjugate, incubate, wash, add substrate, incubate again, add stop solution, and read absorbance at 450nm.
Calculation of Results: Construct a standard curve by plotting absorbance against standard concentrations. Use the curve to determine the concentration of controls and unknown samples.
Example Standard Curve: Provides optical density (OD) readings at 450 nm for various concentrations of Cotinine standards.
See more

Catalog excerpts

Mouse/Rat Cotinine ELISA-1

Mouse/Rat Cotinine ELISA, R5 RC CALBIOTECH A life science company Mouse/Rat Cotinine ELISA Catalog Number: CO096D-100 (96 Tests) INTENDED USE For Research Use Only. Not for use in diagnostic procedures. Cat#: CO096D-100 (96 Tests) For Order and Inquiries, please contact Mil Calbiotech Inc., 1935 Cordell Ct., El Cajon, CA 92020 Tel (619) 660-6162, Fax (619) 660-6970, www.calbiotech.com 1. Distilled or deionized water 3. Disposable pipette tips 4. ELISA reader capable of reading absorbance at 450nm 5. Absorbance paper or paper towel 6. Graph paper STORAGE AND STABILITY 2. Keep microwells sealed in a dry bag with desiccants. 3. The reagents are stable until expiration of the kit. 4. Do not expose test reagents to heat, sun, or strong light. WARNINGS AND PRECAUTIONS 1. For Research Use Only. Not for use in diagnostic procedures. 3. Potential biohazardous materials: The calibrator and controls contain human source components which have been tested and found non-reactive for hepatitis B surface antigen as well as HIV antibody with FDA licensed reagents. However, as there is no test method that can offer complete assurance that HIV, Hepatitis B virus or other infectious agents are absent, these reagents should be handled at the Biosafety Level 2, recommended

 Open the catalog to page 1
Mouse/Rat Cotinine ELISA-2

Mouse/Rat Cotinine ELISA, R5 RC in the Centers for Disease Control/National Institutes of Health manual, "Biosafety in Microbiological and Biomedical Laboratories." 1984 4. Do not pipette by mouth. Do not smoke, eat, or drink in the areas in which specimens or kit reagents are handled. 5. The components in this kit are intended for use as an integral unit. The components of different lots should not be mixed. 6. It is recommended that standards, control and serum samples be run in duplicate. 7. Optimal results will be obtained by strict adherence to this protocol. Accurate and precise pipetting,...

 Open the catalog to page 2
*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.