REFERENCES 1. Agharanya JC. Clinical usefulness of ELISA technique in the assessment of thyroid function. West Afr J Med 1990;9(4):258-63. 2. Baumgartner-Parzer sM; Wagner L; Reining G; Sexl V; Nowotny P; Muller M; Brunner M; Waldh'ausl W. Increase by tri-iodothyronine of endothelin-1, fibronectin and von Willebrand factor in cultured endothelial cells. J Endocrinol 1997;154(2):231 -9. 3. Maes M; Mommen K; Hendrickx D; Peeters D; D'Hondt P; Ranjan R; De Meyer F; Scharp'e S Components of biological variation, including seasonality, in blood concentrations of TSH, TT3, FT4, PRL, cortisol and testosterone in healthy volunteers. Clin Endocrinol (Oxf) 1997; 46(5):587-98. 4. Santini F; Chiovato L; Bartalena L; Lapi P; Palla R; Panichi V; Velluzzi F; Grasso L; Chopra IJ; Martino E; Pinchera A Study of serum 3,5,3'-triiodothyronine sulfate concentration in patients with systemic non-thyroidal illness. Eur J Endocrinol 1996;134(1):45-9 CALBIOTECH A life science company Mouse/Rat Triiodothyronine (T3) ELISA Catalog No. T3043T-100 (96 tests) INTENDED USE For Research Use Only. Not for use in diagnostic procedures. Cat#: T3043T-100 (96 tests) For Order and Inquiries, please contact llJ Calbiotech Inc., 1935 Cordell Ct., El Cajon, CA 92020 Tel (619) 660-6162, Fax (619) 660-6970, www.calbiotech.com MATERIALS NOT PROVIDED 1. Distilled or deionized water 3. Disposable pipette tips 4. ELISA reader capable of reading absorbance at 450nm 5. Absorbance paper or paper towel 6. Graph paper STORAGE AND STABILITY 2. Keep microwells sealed in a dry bag with desiccants. 3. The reagents are stable until expiration of the kit. 4. Do not expose test reagents to heat, sun, or strong light. WARNINGS AND PRECAUTIONS 1. For Research Use Only. Not for use in diagnostic procedures. 3. Potential biohazardous materials: The calibrator and controls contain animal and human source components, which have been tested and found non-reactive for hepatitis B surface antigen as well as HIV antibody with FDA licensed reagents. However, there is no test method that can offer complete assurance that HIV, Hepatitis B virus or other infectious agents are absent. These reagents should be handled at the Biosafety Level 2, as recommended in the Centers for Disease Control/National Institutes of Health manual, "Biosafety in Microbiological and Biomedical Laboratories" 1984.

4. Do not pipette by mouth. Do not smoke, eat, or drink in the areas in which specimens or kit reagents are handled. 5. The components in this kit are intended for use as an integral unit. The components of different lots should not be mixed. 6. It is recommended that standards, control and serum samples be run in duplicate. 7. Optimal results will be obtained by strict adherence to this protocol. Accurate and precise pipetting, as well as following the exact time and temperature requirements prescribed are essential. Any deviation from this may yield invalid data. SPECIMEN COLLECTION HANDLING...