Preserve Cerebrospinal Fluid Leukocytes for 72 hrs to Increase Disease Detection with CSF Sample Storage Tubes The presence of white blood cells in the cerebrospinal fluid (CSF) is indicative of many central nervous system (CNS) diseases. The detection of leukocyte subsets in CSF is of vital importance for the accurate diagnosis and treatment of such diseases. However, low cellularity and cell viability of leukocytes causes challenges when analysing CSF with flow cytometry. This means that samples must be analysed urgently (within 1 h) which is not always possible. TransFix® CSF Sample Storage Tubes provides increased recovery of myeloid and lymphoid cells for up to 72 hours for analysis via flow cytometry, compared to untreated or RPMI-treated CSF samples. Available as in vitro diagnostic devices in Europe. TransFix® allows recovery of leukocytes subsets for up to 72 h days at 2-8°C, maintaining the immunophenotypic profile of CSF for better disease detection. This provides the following advantages: • More accurate diagnosis compared to untreated or RPMI-treated CSF samples • Reduction in the need for repeat lumbar puncture - reduced trauma for patient • Provides the opportunity to transport the samples for centralised diagnosis and multicentre clinical studies • Greater efficiency in testing - allows for batching of samples prior to testing • Further tests can be performed on the same sample after the initial analysis, without subject recall • Easy to use - just transfer the CSF sample into the tube within 1 hour of lumbar puncture and mix by inversion • Eliminates the need for weekend and evening work • Reduces the impact of unexpected machine breakdown or staff shortages Widely endorsed by the clinical community Recommended in the guidelines published in the British Journal of Haematology (1,2) • The European Society for Clinical Cell Analysis and the Italian Society for Clinical Cell Analysis (3) ) • The Spanish Lymphoma Group (4) • The Spanish Group for the Study of CNS Disease in Non-Hodgkins Lymphoma (5) | • The Brazilian Group of Flow Cytometry (6) • International Working Group on Flow Cytometric Immunophenotyping of Cerebrospinal fluid (7) ORDER NOW! Visit: www.caltag.co.uk/distributors to find your local distributor

Mock CSF was stabilised with TransFix®, basal RPMI and complete RPMI and compared to untreated mock CSF (control). Figure 1 shows the recovery of lymphocyte subsets and monocytes at each time point. The event numbers for each cell subpopulation in the TransFix®-treated sample remained relatively consistent for at least 72 hours whereas the event numbers in all 3 other samples were low, particularly within the first hour. Cell events also decreased sharply in non-TransFix® treated samples after 24 hours. Legend | Control H TransFix Complete RPMI Figure 2. Side scatter vs CD45+ dot plots for the...