Instrumental Thin-Layer Chromatography
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INSTRUMENTAL THIN-LAYER CHROMATOGRAPHY WORLD LEADER IN PLANAR CHROMATOGRAPHY

CAMAG – Your partner in all fields of Planar Chromatography CAMAG has the tradition of serving planar chromatography since 1961. We develop and manufacture sophisticated instruments and associated software for the state-of-the-art analytical technique. Our products are marketed directly in Switzerland, through daughter companies in Germany and in the USA, and through carefully selected distributors worldwide. We see ourselves as a flexible, customer friendly, science based company that has made its mark as a reliable partner in all branches of planar chromatography. We provide competent...

Steps of the TLC/HPTLC procedure Sample Application The samples are applied onto the plate either as spots or as bands. Precision of the applied volume, exact positioning and compactness of application zone determine the quality of the final result. Chromatogram Development The mobile phase is drawn through the stationary phase by capillary action. Samples are separated into their components which remain in their position on the layer after the mobile phase has been evaporated. Chromatography is affected by the gas phase in the chromatographic chamber. Substances without chromophores or...

What is offered by CAMAG? Nanomat 4 Automatic TLC Sampler 4 (ATS 4) Linomat 5 Page 7 Page 8 Page 10 Sample Application Developing Chambers smartAlert, smartCut Automatic Developing Chamber (ADC 2) AMD 2 System Automated Multiple Development HPTLC Vario System Page 14 Page 15 Page 16 Page 17 Page 18 Chromatogram Development Chromatogram Immersion Device 3 TLC Sprayer Derivatizer TLC Plate Heater 3 TLC Spray Cabinet 2 Page 21 Page 21 Page 22 Page 24 Page 24 UV Lamp 4, UV Cabinet 4 TLC Visualizer 2 BioLuminizer 2 TLC Scanner 4 TLC-MS Interface 2 Page 26 Page 28 Page 30 Page 32 Page 34...

High-Performance Thin-Layer Chromatography The high performance version of planar chromatography distinguishes the technique. HPTLC comprises the use of chromatographic layers of utmost separation efficiency and the employment of state-of-the-art instrumentation for all steps in the procedure, precise sample application, standardized reproducible chromatogram development and software controlled evaluation. Of course, conventional TLC, manually performed und using inexpensive equipment still has its place in almost all laboratories as a convenient tool for simple and rapid separations. HPTLC...

Sample Application Sample application determines quality and reproducibility of the analysis

Sample Application – Variations Application of samples, reference standards and/or reference materials is the first step in the workflow of TLC/HPTLC and it affects significantly the quality of the result at the end of the process. The choice of the application technique and the device depend on the requirements of precision, sample volumes, number of analyses and the desired grade of automation. Spotwise sample application using a fixed volume capillary is the simplest way. Sample volumes of 0.5 to 5 μL can be applied as spots onto conventional layers without intermediate drying, on HPTLC...

CAMAG® Nanomat 4 and Capillary Dispenser The Nanomat 4 serves for easy application of samples in the form of spots onto TLC and HPTLC layers, precisely positioned and without damage to the layer. The actual sample dosage is performed with a disposable capillary pipette, which is precisely guided, thus ensuring that the chromatogram can be scanned automatically according to a programmed pattern. Capillary pipettes The capillary pipettes are loaded into the dispenser in magazines. Capillaries of 0.5, 1.0, 2.0, and 5.0 µL volume are available. Each capillary size requires an appropriate...

CAMAG® Automatic TLC Sampler 4 (ATS 4) Automatic sample application is a key factor for productivity of the HPTLC laboratory. The ATS 4 offers fully automatic sample application for qualitative and quantitative analyses as well as for preparative separations. It is suited for routine use and high sample throughput. Samples are either applied as spots through contact transfer (0.1–5 µL) or as bands or rectangles (0.5 to > 50 µL) using the spray-on technique. Starting zones in the form of narrow bands offer the best separation attainable with a given chromatographic system. Application in the...

Operation of the ATS 4 with visionCATS Precise sample application is a crucial factor for the quality of the HPTLC analysis and the results obtained. When using visionCATS HPTLC software with its easy to navigate user interface to control the ATS 4, a fully automated sample application for routine use and high sample throughput is supported. The dialog box for instrument parameters offers user-friendly default combinations. For instance, the user can select the solvent type most similar to the solvent actually used. The software will then automatically adapt the instrument defaults to...

CAMAG® Linomat 5 With the Linomat 5 samples are sprayed onto TLC/HPTLC plates in the form of bands with nitrogen or compressed air. Sample application is automatic, only changing the syringe (filling, inserting and rinsing) is manual. The Linomat is suitable for small sample throughput. Software-controlled operation with visionCATS Operation in stand-alone mode The Linomat 5 is controlled by visionCATS, the new generation of HPTLC software. Instrument handling is now easier and more convenient. Simply select a plate format, fill in the sequence table and start working. With visionCATS, the...

Chromatogram Development Chromatogram development under reproducible standardized conditions is a key to the quality of the result

Chromatogram Development TLC and HPTLC differ from all other chromatographic techniques in the fact that in addition to stationary and mobile phases, a gas phase is present. This gas phase can significantly influence the result of the separation. The following considerations primarily concern silica gel as stationary phase and a process usually described as adsorption chromatography. In the developing chamber four partially competing processes occur: 1 Between the components of the developing solvent and its vapor, an equilibrium will be established gradually. This process is called chamber...