BUFFERS AND SOLUTIONS
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PACKAGE SIZES – SUMMARY LowCross-Buffer® Antibody Stabilizer “TRIS” Sample Buffer Stripping Buffer Liquid Plate Sealer® Washing Buffer “PBS” 10× 500 mL order number 140 500 Washing Buffer “PBS IHC” 10× 500 mL order number 141 500 Washing Buffer “TRIS” 10× 500 mL order number 145 500 Washing Buffer “TRIS IHC” 10× 500 mL order number 146 500 Starter Package small Starter Package “IHC” small CANDOR Bioscience GmbH Simoniusstrasse 39 support@candor-bioscience.de General inquiries Blocking Sampler

CONTENT Immunoassays are an important tool in everyday work for many scientists and lab technicians. But regardless whether we are experts in drug discovery, medical research, veterinary diagnostics, biotechnological quality control, environmental analysis or whatsoever - we do not perform immunoassays only because it is a funny amusement. We just and only perform immunoassays to get the correct answer to our specific scientific questions. If this is the case for you, what is your opinion? Shouldn't all of your assay results be accurate? Yes. We do think so. And that is the reason why our...

Linked Immuno Sorbent Assay. The Western blotting technique is Protein arrays are miniaturized assays Immuno-PCR combines ELISA tech- mixtures. First, the proteins are sep ELISA is the abbreviation for Enzyme- structures are stained by using anti- allel. They use proteins or antibodies used to detect proteins from crude arated by gel-electrophoresis into beled specific antibody to the target bands. Then all proteins are trans antigen (called analyte). The signal of ferred to a membrane, where the the enzyme labeled antibody is the target protein band is detected with conversion of a...

OPTIMIZERS Optimizers are a class of advantageous and multifunctional solutions for immunoassays. They are outstanding compared to other diluents and buffers. LowCross-Buffer® is the first solution that combines several functionalities of diluents in one product and is not only an assay diluent. Additionally it can reduce HAMA-interference and furthermore helps to avoid matrix effects. LowCross® HRP-Stab is another product with several functionalities. This product enables convenient one step incubation assays without negatively affecting the reliability of the results. This is due to both...

Western Blot Antibody and sample diluent for In addition matrix effects, coming for cross-reactivities and matrix effects That means that matrix effects example from blood sera or plasma no longer exacerbate detection of Buffer® helps to reduce interference analytes. Minimization of all these negative effects upgrades the quality improved. Nonspecific binding of formulation immunoassays. 1:750 LowCross-Buffer® 1:750 PBS/BSA Tween the antibodies, negative effects of interfering substances and low or medium affinity cross-reactivities Analyte concentration [ng/mL] Calibration curve of an...

BLOCKERS The surfaces of ELISA plates or Western blotting membranes are optimized for efficient attachment of proteins and antibodies. This enables optimal assays with a high density of capture antibodies or capture molecules on the surfaces. As a consequence of this optimization of the surfaces, many other proteins or peptides and other molecules from the sample can attach on the surface, too. Without surface blocking, assay antibodies, tracers, analytes or other components of the sample would bind to the surface. This can lead to false results or high background s ignals and therefore it...

Western Blot Casein based blocker for minimizing nonspecific binding to surfaces. Additionally, no components of the sample can bind nonspecifically to The Blocking Solution is based on membranes or other protein binding chemically modified and fragmented surfaces and prevents nonspecific The Blocking Solution saturates free highly purified casein. This minimizes unwanted binding of Blocking Solution has been developed the analyte or the detection antibody and up-scaled by CANDOR. This proprietary process guarantees an extremely good lot-to-lot consistency which can normally be obtained...

Western Blot BSA-free blocker for saturating free other protein binding surfaces. e.g. for diagnostics, pharmaceutical research, peptides were chemically modified pharmaceutical process nonspecific binding even when using Blocking Solution. Protein Array BSA-Block is a well-established, versatile blocker for many applications. BSA-Block, we recommend using The Protein Array binding sites on plastic surfaces or SmartBlock™ is a modern blocker on Standard blocker for saturating free other protein binding surfaces. priced assays with good reliability binding sites on plastic surfaces or...

STABILIZERS Immunoassay stabilizers are needed to preserve the native conformation of proteins and to protect them from losing their functionality. Stabilizers for immunoassays allow long-term storage of antibodies, conjugates (e.g. HRP-labeled or AP-labeled) and antigens in solutions as well as coated onto surfaces like microtiter plates or others. CANDOR offers stabilizers to protect proteins, antibodies and conjugates in solution as well as stabilizers for antibodies coated on surfaces. The portfolio ranges from Antibody Stabilizer over HRP-Protector™ and LowCross® HRP-Stab to Liquid...

Western Blot Assay diluent for long-term storage HRP-Stab gives peroxidase-antibody nonspecific binding, cross-reactivities stability over several years, even when and matrix effects in immunoassays. LowCross® reliability conjugates the crucial long-term stored as a ready-to-use solution. Conjugate activity [%] The result is an assay diluent and storage conjugates in one solution with high derived ones. The LowCross-effect interferences minimizes effects, cross- ready-to-use dilution. reactivities and nonspecific binding. Protein Array Thus several washing steps like in sequential assays...