LowCross® HRP-Stab
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LowCross® HRP-Stab Assay diluent for long-term storage of HRP conjugates and for minimizing nonspecific binding, cross-reactivities and matrix effects in immunoassays Storage: contains < 0.0014 % [w/w] reaction mass of CMIT/MIT (3:1) Expiry date when stored unopened: see label on the bottle For general laboratory use Instructions for use LowCross® HRP-Stab is ready-to-use. Please shake the buffer thoroughly before use. Conjugates can be diluted directly in LowCross® HRP-Stab at end concentrations. Typical conjugate concentrations are in the range of 40 to 500 ng/ml. LowCross® HRP-Stab can be used directly as assay buffer in immunoassays. Appearance of signal reduction: The LowCross®-effect suppresses low and medium affinity binding events. As a consequence, a slight signal reduction may occur if polyclonal antibodies (which generally also contain low- and medium-affinity binding components) are used. In this case, the amount of high-affinity antibodies can be raised by moderately increasing the antibody concentration in order to achieve the desired signal strength again. The unwanted low and medium-affinity binding will remain suppressed by the LowCross®-effect. When using low- or medium affinity monoclonal antibodies, signal deletion may occur as the LowCross®effect completely suppresses their binding. We recommend the use of suitable high-affinity antibodies. The suitability of LowCross® HRP-Stab for the respective assay and the respective conjugates must be tested by the user. Stability data of one peroxidase conjugate cannot be directly transferred to other conjugates. Therefore, each conjugate must be tested for its shelf-life in LowCross® HRP-Stab. If LowCross® HRP-Stab is used for immunodiagnostic kits, the shelf life has to be tested according to the applicable regulatory requirements for diagnostics. Please note that high protein concentrations and/or microbial contamination may reduce the effectiveness of the preservative. If you add antibodies/conjugates for storage in a non-sterile manner and you are unsure about potential microbial contamination, it may be beneficial to add additional preservative or also antibiotics. For further information please visit www.candor-bioscience.com. LowCross is a registered trade mark of CANDOR Bioscien