qMAXSen™ One-Step RT-qPCR Kit
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Data sheetqMAXSen™ One-Step RT-qPCR Kit[ Coronavirus Detection (Strain 2019-nCoV)] Cat. No: E01560 (100 rxns) Each kit contains: A novel coronavirus, currently termed 2019-nCoV, was announced as the etiological agent of cases of ongoing pneumonia outbreak in Wuhan City. This kit describes the use of real time RT-PCR for the in vitro detection of 2019-nCoV in respiratory specimens (sputum; nasopharyngeal, oropharyngeal aspirates, washes or swabs; tracheal aspirates). qMAXSen™ One-Step RT-qPCR Kit allows efficient cDNA synthesis and Real-Time PCR in a single tube. The kit includes a qPCR master mix supplied in a 2X concentration to perform real-time PCR. The 2X qPCR Master Mix contains all the reagent (except PCR primers, probe and template) needed for running PCR reactions. In addition, a separate RT mix that comprises a balanced mixture of both RTase (Reverse Transcriptase) and RNase Inhibitor is also provided. Also, the kit contain one sets of primers and fluorescent probes to differentiate between 2019-nCoV and SARS-CoV (or bat SARS related CoV). The probes are readout in different channels. Coronavirus 2019-nCoV RNA targets are amplified and detected in the FAM channel, Coronavirus SARS-CoV (or bat SARS related CoV) RNA targets are amplified and detected in the HEX, VIC or JOE channel (depending on the equipment used). The assay includes positive control (PTC) and negative control (NTC). The positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a negative control reaction should be included every time the kit is used. ✓ 2X qPCR Master Mix (1 vial) ✓ RT mix (1 vial) ✓ PCR Primer/ Probe set (1 vial) 12 ✓ Rehydration Buffer (1 vial) ✓ COVID-19 Positive control (PTC) (1 vial) ✓ Non-Template Control (NTC) (1 vial) Storage qMAXsen™ One-Step RT-qPCR Kit is shipped on dry/blue ice. The Kit should be stored at -20°C upon receipt. Avoid repeated freezing and thawing. Maintain cold when thawed. *PCR Primers/ Probe Set must be rehydrated in the dark. Mix gently and aliquot in different tubes. Store aliquots at -20°C. Features ✓ Inclusivity: SARS like coronavirus and specific detection of 2019-nCoV ✓ Dual Color Multiplex Assay Format Product use limitation In response to the new coronavirus (2019-nCoV) emergency, this kit is intended to facilitate and support research. This kit is a method of assistant diagnosis for research use only and cannot be used as a basis for confirming or excluding cases. Research Use Only (RUO). This kit has not been tested and validated by any public health agency. PCR Primer/ Probe set is referenced in publicly available 2019-nCoV real-time PCR protocols (information can be found on the WHO website). Canvax Biotech, S.L. C/Astronoma Cecilia Payne. Edif. Canvax. 14014 Cordoba, Spain. ® : +34 957 348 066

BASIC PROTOCOL 1. Thaw kit components on ice. Mix each solution well. The following protocol is recommended for a 20 pl reaction volume: 2. Set up the following reaction mixture. *Acquisition must be performed at the end of this stage 6. Select the fluorescent channel (FAM/ HEX) of instrument for testing. 2019-nCoV = FAM (465-510) SARS-CoV (or bat SARS related CoV) = VIC / HEX / JOE (533-580) Canvax Biotech, S.L. C/Astronoma Cecilia Payne. Edif. Canvax. 14014 Cordoba, Spain. ® : +34 957 348 066

Analysis of results Follow instrument software instructions to generate cycle threshold (Ct) values from the acquired data. Controls ✓ Non-Template Control (NTC) should be negative and not exhibited Ct value in FAM channel and HEX channel. If NTC reaction is positive, sample contamination has been occurred. ✓ Positive Template Control (PTC) should be positive and exhibited an expected Ct value for each channel included in the kit. If these results are not obtained, repeat the assay implementing corrective actions for failed reactions. Samples When all controls exhibit the expected...