Comparison of Ibidi and Traditional 96-Well Plates for Cell Counting

Overview

This document compares the advantages of using Ibidi plates versus traditional clear 96-well plates specifically for cell counting applications.

Key Specifications and Differences

• Sample Volume: Ibidi plates require smaller volumes (10 µL to 50 µL) compared to traditional plates.
• Well Dimensions: Ibidi wells have a height of 0.1 mm, significantly thinner than the 0.8 mm height of traditional wells, allowing for more precise imaging and counting.
• Chamber Size: Both Ibidi and traditional hemocytometers have a 3 mm chamber size, but Ibidi's design optimizes volume capture.

Performance Comparison

A comparison of live Peripheral Blood Mononuclear Cells (PBMC) counts was conducted using Ibidi plates and a standard 2-chamber hemocytometer across 12 samples.

• The data indicates that Ibidi plates provide comparable live cell counts to the traditional hemocytometer method.
• The captured volume and imaging quality with Ibidi plates enhance counting accuracy and efficiency.

Advantages of Ibidi Plates

• Reduced sample volume requirements, conserving precious samples.
• Improved imaging due to thinner well height, facilitating better cell visualization.
• Compatibility with standard microscopy cameras for efficient data capture.

Summary

Ibidi plates offer a practical and efficient alternative to traditional 96-well plates and hemocytometers for cell counting, especially when working with limited sample volumes. Their design enhances imaging quality and maintains accuracy in live cell counting.