THE ELISPOT Source Elevating T Cell Multiplexing to New Heights with Four Color FluoroSpot

Product catalog summary

Overview: The document discusses optimized ELISPOT/FluoroSpot assays designed to detect antigen-specific T cells at very low frequencies within peripheral blood mononuclear cells (PBMC). These assays are crucial for identifying single analyte-secreting T cells among a million bystander cells.

Specifications: The assays offer a comprehensive portfolio of multicolor ELISPOT kits, including two-, three-, and four-color T cell kits. These kits are designed to economize cell utilization and enable the detection of polyfunctional T cells.

Procedures: The ImmunoSpot® Kits include low auto-fluorescent plates, serum-free media, antibodies, and fluorescent conjugates with separate emission spectrums. This setup allows for the detection of up to four cytokines, simplifying and speeding up the assay process.

Norms and Standards: All ImmunoSpot® Kits are quality-controlled to meet high sensitivity thresholds, capable of detecting a single secreting cell within a million bystander cells. The kits are designed to minimize common assay problems such as unspecific enhancement or suppression.

Recommendations: The document recommends using CTL-Test™ Medium, a standardized serum-free alternative, to reduce variability in test results. It also suggests using ePBMC® Reference Sample QC Sets™ to minimize intra- and inter-laboratory variability.

Key Features: Double-Color Enzymatic Kits come with two substrates, CTL-TrueBlue™ and CTL-TrueRed™, which allow for precise analyte quantification and accurate data analysis. The kits are designed to prevent overdevelopment and enable fixed development times.

Product Range: The document lists various single-color and multicolor kits available for human, non-human primate, mouse, and porcine samples. These include enzymatic and fluorescent kits with pre-coated plates available for some options.

Comparison with Competitors: ImmunoSpot® Kits offer several advantages over competitors, including options for 96-well and 384-well plates, low auto-fluorescence plates, and serum-free media. They also provide comprehensive scientific and technical support.

Contact Information: The document provides contact details for Cellular Technology Limited, including email, website, and phone numbers.

Catalog excerpts

THE ELISPOT Source Elevating T Cell Multiplexing to New Heights with Four Color FluoroSpot-1

THE ELISPOT Source Elevating T Cell Multiplexing to New Heights with Four Color FluoroSpot ptimized ELISPOT/FluoroSpot assays can reliably detect antigen-specific T cells even if they occur in frequencies of 0.0001% within PBMC. That’s a single analyte-secreting T cell that can be reliably identified within one million bystander cells. To further increase the use of precious cell material, we offer the most comprehensive portfolio of multicolor ELISPOT kits available on the market, including two-, three-, and four-color T cell kit. Multiplexing analytes not only helps economize cell utilization but it also enables the detection of polyfunctional T cells. Polyfunctional cells are easily detected with ImmunoSpot® Software. The reagents included in the fluorescent ImmunoSpot® Kits have been tailored to shorten and simplify the assay procedure. Kits contain low auto-fluorescent plates, diluents, serum-free media, antibodies, and most importantly, fluorescent conjugates that have separate emission spectrums to easily and accurately detect up to four cytokines. ELISPOT assays have never been faster and easier

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THE ELISPOT Source Elevating T Cell Multiplexing to New Heights with Four Color FluoroSpot-2

Multiplexing T Cell FluoroSpot and ELISPOT ELISPOT Source The First Step in Multiplexing: Double-Color Kits CTL is taking multiplexing of T cell assays even further with the only double-color enzymatic ELISPOT Kit on the market that can reliably and accurately count polyfunctional cells. ELISPOT is known to be able to detect a single secreting cell within a million bystander cells. All ImmunoSpot® Kits, human, non-human primate, mouse, and porcine kits, are quality-controlled to meet this exquisite sensitivity threshold. 52 Polyfunctional T cells secreting both IFN-γ and IL-2. IFN-γ spots counted...

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THE ELISPOT Source Elevating T Cell Multiplexing to New Heights with Four Color FluoroSpot-3

NHP IFN-y / IgG / IgA IFN-y / IgG / IgM IL-6 / IgG / IgA IL-6 / IgG / IgM IL-10 / IgG / IgA IL-10 / IgG / IgM TNF-a / IgG / IgA TNF-a / IgG / IgM PORCINE ifn-y / IgA

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THE ELISPOT Source Elevating T Cell Multiplexing to New Heights with Four Color FluoroSpot-4

ImmunoSpot® Kits versus the Competition 20521 Chagrin Boulevard +1 216-791-5084 [email protected] Shaker Heights, +1 216-751-1373 Fax www.immunospot.com The Immune Monitoring Company Cleveland, OH 44122-5350 USA +1 888-791-4005 Toll Free US The CTL logo, the spot cloud, and the trademarks ePBMC® and Fully Functional PBMC at your Fingertips™ are property of Cellular Technology Limited. All rights reser

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