THE ELISPOT Source Elevating T Cell Multiplexing to New Heights with Four Color FluoroSpot ptimized ELISPOT/FluoroSpot assays can reliably detect antigen-specific T cells even if they occur in frequencies of 0.0001% within PBMC. That’s a single analyte-secreting T cell that can be reliably identified within one million bystander cells. To further increase the use of precious cell material, we offer the most comprehensive portfolio of multicolor ELISPOT kits available on the market, including two-, three-, and four-color T cell kit. Multiplexing analytes not only helps economize cell utilization but it also enables the detection of polyfunctional T cells. Polyfunctional cells are easily detected with ImmunoSpot® Software. The reagents included in the fluorescent ImmunoSpot® Kits have been tailored to shorten and simplify the assay procedure. Kits contain low auto-fluorescent plates, diluents, serum-free media, antibodies, and most importantly, fluorescent conjugates that have separate emission spectrums to easily and accurately detect up to four cytokines. ELISPOT assays have never been faster and easier

Multiplexing T Cell FluoroSpot and ELISPOT ELISPOT Source The First Step in Multiplexing: Double-Color Kits CTL is taking multiplexing of T cell assays even further with the only double-color enzymatic ELISPOT Kit on the market that can reliably and accurately count polyfunctional cells. ELISPOT is known to be able to detect a single secreting cell within a million bystander cells. All ImmunoSpot® Kits, human, non-human primate, mouse, and porcine kits, are quality-controlled to meet this exquisite sensitivity threshold. 52 Polyfunctional T cells secreting both IFN-γ and IL-2. IFN-γ spots counted...

NHP IFN-y / IgG / IgA IFN-y / IgG / IgM IL-6 / IgG / IgA IL-6 / IgG / IgM IL-10 / IgG / IgA IL-10 / IgG / IgM TNF-a / IgG / IgA TNF-a / IgG / IgM PORCINE ifn-y / IgA

ImmunoSpot® Kits versus the Competition 20521 Chagrin Boulevard +1 216-791-5084 [email protected] Shaker Heights, +1 216-751-1373 Fax www.immunospot.com The Immune Monitoring Company Cleveland, OH 44122-5350 USA +1 888-791-4005 Toll Free US The CTL logo, the spot cloud, and the trademarks ePBMC® and Fully Functional PBMC at your Fingertips™ are property of Cellular Technology Limited. All rights reser