NC-250

NC-250

NC-250

Product catalog summary
Overview of NucleoCounter® NC-250™
The NucleoCounter® NC-250™ is a compact, benchtop fluorescence microscope designed for fast and precise cell counting and analysis. It features dual fluorescence channels for detecting signals from cells stained with fluorescent dyes, making it suitable for various applications including vaccine production, stem cell research, and toxicology.

Key Features
  • Processes up to 8 samples in one run, providing results in less than 3 minutes.
  • Performs total cell count, viability, cell diameter measurement, and percentage of aggregated cells.
  • Offers high precision cell cycle analysis and quick apoptosis assays using VitaBright-48™ stain.
  • Automated PDF reporting and compliance with 21 CFR Part 11.
  • No calibration or maintenance required, ensuring excellent reproducibility.

Specifications
  • Loading volume: 30 μl for A2-slides, 10 μl for A8-slides.
  • Measurement volume: 11.2 μl for A2-slides, 2.4 μl for A8-slides.
  • Measurement range: 5 x 103 to 1 x 107 cells/ml, optimal range: 5 x 104 to 5 x 106 cells/ml.
  • Size: 19 x 29 x 32 cm, weight 9 kg.
  • Software: NucleoView™ NC-250 software.

Assays
  • Viability and Cell Count Assay: Mix cell sample with staining solution, load slide, and press RUN for results in 3 minutes.
  • Cell Cycle Assay: Incubate with staining solution and lysis buffer, neutralize, load slide, and press RUN for profiles in 4 minutes.
  • Vitality Assay: Add VitaBright-48™, load slide, and press RUN for apoptosis measurements in 4 minutes.

For more information, visit www.chemometec.com/NC-250.
See more

Catalog excerpts

NC-250-1

NucleoCounter® NC-250™ The Fast and Precise Cell Counter NucleoCounter® NC-250™ NucleoCounter® NC-250™ is an integrated fluorescence microscope with dual fluorescence channels designed to detect signals from cells stained with fluorescent dyes. Performing multiple determinations of viability and cell count is fast and easy with the NucleoCounter® NC-250™. Process 8 samples in one run. Just prepare your cell suspensions by adding the staining solution containing fluorescent dyes, load the 8 cell samples into one slide and press RUN. of the NucleoCounter® NC-250™  8 viability and cell counts in less than 3 minutes - Total cell count - Viability - Cell diameter - % of aggregated cells  Up to 8 advanced analyses in one run The NucleoCounter® NC-250 ™ also provides easy, fast (5 minutes) and high precision assaying of cell cycle profiles. - High precision cell cycle analysis - Cell health determination with the fast apoptosis assay In addition, you can measure cell health with our quick and reliable apoptosis assay. VitaBright-48 ™ stain instantly becomes fluorescent when it binds to thiols inside cells. The level of thiols is a direct measurement of cell health. A reduction in the level of reduced thiols in a cell is a documented event in apoptosis.  Fast and easy operation The NucleoCounter® NC-250 ™ is a compact benchtop instrument which fits perfectly into any cell laboratory.  Excellent reproducibility  No calibration  Maintenance and service free  Automated PDF reports Vaccine Production | Microcarriers | Stem Cells | Cancer Cells | C

 Open the catalog to page 1
NC-250-2

NC-250™ Three useful Assays Viability and Cell Count Assay Viability and Cell Count Assay Mix cell sample with the staining solution, load the slide and press RUN. Get 8 results in 3 minutes with total cell count, viability, cell diameter and percentage of aggregated cells. Cell Cycle Assay Cell Cycle Assay Incubate cell sample for 5 minutes at 37°C with a mixture of staining solution and lysis buffer, neutralize by adding stabilizing buffer, load slide and press RUN. Get 8 high precision cell cycle profiles in 4 minutes. Vitality Assay Vitality Assay Add VitaBright-48™ to your sample, immediately...

 Open the catalog to page 2

All Chemometec catalogs and technical brochures

  1. SCC-400

    2  Pages

  2. SCC-100

    2  Pages

  3. NC-200

    2  Pages

  4. SCC- 400

    2  Pages

  5. SCC-10 0

    2  Pages

  6. YC-100

    2  Pages

  7. NC-100

    2  Pages

  8. NC-3000

    6  Pages

*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.