NC-3000™ Vitality Assay – For easy detection of changes in the cellular level of reduced thiols The vitality assay provides easy determination of the level of thiols, such as reduced glutathione (GSH). GSH is the most abundant low molecular weight thiol in animal cells; thus its oxidation status largely determines the thiol-disulfide status of the cell by thiol-disulfide interchange reactions. GSH is involved in many cellular processes including quenching of free radicals, drug detoxification, cell signaling, and cell proliferation. The decrease in cellular GSH concentration is an early hallmark in the progression of cell death in response to different apoptotic stimuli in many cell types. Key Benefits of the NC-3000™ Vitality Assay Automated detection of changes in the level of reduced thiols!  Extremely easy assay for evaluation of cellular health, oxidative stress and indirect detection of apoptosis  Total assay time less than one minute  Acquisition and analysis at the single cell level in one simple step Vitality Assay  User friendly protocol with predefined settings  PlotManager for superior data presentation Image Cytometry TIME TOTAL ASSAY LESS THAN  Export of data in FCS/ACS formats The NucleoCounter® NC-3000™ - Next generation cell analysis FIXED ASSAYS HIGH SPEED CELL COUNT FAST ANALYSIS VISUAL INSPECTION LEARN MORE Viability and Cell Count | Cell Cycle Assays | Caspase 3/7, 8 & 9 | Annexin V | Mitochondrial Potential | Cell Vitality | DNA Fragmentation | GFP Transfection Efficiency | FlexiCyte™ (u

Scatter plots and histograms showing thiol level distribution (VB-48™ intensity) in the cell population are automatically displayed after analysis. Reduced Thiols By comparing the VB-48™ intensity of treated cells and control cells the fraction of cells with low vitality (e.g. apoptotic or stressed cells) can be determined. Image acquired with the NucieoCountef* NC-3000™ Automated PDFreports Hybridoma cells were grown in the absence (upper row) or in the presence (lower row) of etoposide. Cells were stained with VB-48™, Acridine Orange (AO) and Propidium Iodide (PI) and analysed using the Vitality...