Introduction
CHROMagar Y.enterocolitica is a specialized culture medium designed for the detection and differentiation of pathogenic Yersinia enterocolitica strains. It offers high specificity and selectivity, allowing for clear differentiation between pathogenic and non-pathogenic strains.

Specifications
The medium is characterized by its ability to differentiate pathogenic Y.enterocolitica, which appear mauve, from non-pathogenic strains and other background flora, which appear metallic blue or are inhibited. This differentiation is crucial as traditional media like CIN agar do not distinguish between these strains effectively.

Performance
CHROMagar Y.enterocolitica reduces the background flora significantly, making it easier to identify suspect colonies with pathogenic potential. This is particularly important as Y.enterocolitica is a common foodborne pathogen, often found in pigs, and can grow at refrigeration temperatures, posing a food safety risk.

Usage Instructions
The medium is used by direct streaking or after an enrichment step, with incubation at 30°C for 36 hours under aerobic conditions. The product comes in a 5000 ml pack, including a powder base and a supplement, with a shelf life of 2 years for the base and 18 months for the supplement.

Quality Control
Quality control strains include Y.enterocolitica pYV+ (mauve), Y.enterocolitica pYV- (metallic blue), and other inhibited strains like E.coli and Pseudomonas.

Conclusion
CHROMagar Y.enterocolitica provides a reliable and efficient method for the detection and differentiation of pathogenic Y.enterocolitica, reducing false positives and focusing laboratory resources on truly pathogenic strains.