MAGNETIC BEAD BASED ISOLATIONS FROM LARGE VOLUMES OF BLOOD Description The Clean Blood LV DNA Kit has been designed for the isolation of high- quality DNA from up to 10 mL of whole blood or saliva samples. The isolation procedure allows for a fast and robust genomic DNA isolation of up to 24 samples in less than 2,5 hours. By combining CleanNA's magnetic particles with our propriety buffer system, the genomic DNA is efficiently bound to our magnetic particles once the cells in the whole blood or saliva sample have been lysed. Optimized for outstanding performance in higher volumes, the CleanNA particles allow for faster separation times, resulting a shorter process and a higher yield. Our Clean Blood LV DNA Kit's buffer system minimizes the binding of contaminants and once bound to our magnetic particles, purifies the DNA in a series of rapid washing steps. Finally, the genomic DNA is recovered from the magnetic particles using an elution buffer and is directly suitable for use in most downstream applications. Downstream Applications • Sanger Sequencing Features & Benefits • High quality DNA • Fast and Efficient protocol • Scalable for isolation of various sample volumes • Adaptable to many automated liquid handling workstations on the market • Compatible with anticoagulants such as EDTA, Citrate and Heparin Coenecoop 75 2741 PH Waddinxveen The Netherlands [email protected] @) www.cleanna.com

Human genomic DNA has been isolated from 5 and 10 mL whole blood samples (EDTA). The DNA was eluted in 1 and 2 mL volume using CleanNA's elution buffer (10 mM Tris-HCl; pH 8.0). Both yield and purity have been determined using the Denovix DS-11 spectrophotometer. Clean Blood LV DNA Kit provides high molecular weight DNA Human Genomic DNA has been isolated from a total of 8 samples, 10 mL of whole Blood (EDTA) using the Dynamic Devices LYNX 900. Isolated genomic DNA was analyzed using the Agilent TapeStation. Real-Time PCR inhibition data Sample ID's □ Undiluted D10 fold dilution D100 fold dilution...