CleanNGS

Product catalog summary

Introduction
CleanNGS is a product designed for the purification and size selection of DNA and RNA in next-generation sequencing (NGS) libraries. It has been a standard in the field since 2005, offering high-quality nucleic acid preparation essential for accurate NGS data.

Specifications and Features
CleanNGS utilizes a special buffer formulation and high-quality magnetic beads to ensure optimal size selection and efficient DNA and RNA recovery. It is manufactured RNase-free, making it suitable for both DNA and RNA workflows. The product is also available in a CE-IVD marked version, CleanNGS Dx, for diagnostic applications.

Benefits

Suitable for NGS DNA and RNA
Easy automation
Fast and efficient
CE-IVD marked for diagnostic procedures

Applications
CleanNGS is used for NGS library purification and double-size selection. The CleanNGS Dx version is applicable in diagnostic workflows.

Performance and Comparisons
Figures 1 and 2 demonstrate CleanNGS's double-size selection capabilities, showing consistent performance comparable to competitors. CleanNGS efficiently excludes fragments outside target cutoff regions.

Workflow
The workflow involves adding CleanNGS reagent with magnetic beads to the NGS library solution, binding large DNA fragments, and using a magnet to clear them. Smaller fragments are then bound and purified, ready for downstream sequencing.

Performance Evaluation
Figures 3, 4, and 5 illustrate CleanNGS's performance in sequencing quality, recovery percentage, and RNA quality, respectively. CleanNGS shows equal or superior performance compared to competitors, with high recovery rates and no RNA degradation.

Ordering Information
CleanNGS is available in various sizes, with specific part numbers for ordering. The product is distributed by CleanNA, which provides reliable nucleic acid isolation solutions.

Contact Information
For orders and inquiries, contact CleanNA at their Netherlands office or via their website.

Catalog excerpts

DNA and RNA cleanup Dx CE-IVD version also available Purification and size selection of next-generation sequencing libraries

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A new standard for DNA & RNA library purification Since its introduction in 2005, Next-Generation Sequencing (NGS) has revolutionized translational genomics and molecular diagnostics by enabling the high-throughput decoding of DNA and RNA. A critical step in generating high-quality NGS data is the preparation of pure nucleic acids of specific lengths. Our flagship product CleanNGS is already well-established in NGS purification and library size selection workflows across the globe. Our special buffer formulation ensures optimal size selection for NGS libraries and together with our highquality...

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Starting material Beads bind fragments Magnet holds bound fragments Transfer supernatant Beads bind fragments and discard supernatant Wash away polution Elute fragments from beads and discard supernatant In Figure 3 CleanNGS and two other commercially available kits (I and A) were used for purification and double-size selection in a Nextera DNA Flex Library Prep. The generated DNA libraries were sequenced using an Illumina MiSeq and the quality scores of the Read 1 and Read 2 traces were determined. CleanNGS demonstrated equal performance versus the competitor products. Figure 4 compares percentage...

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Ready to order? Order via your local distributor or contact us via our details below. Order info About CleanNA CleanNA advances human health with reliable nucleic acid isolation solutions for life science and healthcare labs. Our portfolio includes research and CE-IVD diagnostic products. Our magnetic bead-based reagents are specifically designed to be used in automated laboratory workflows. Part number Part number Pack size Part number Clean Magnet Plate 96-Well CleanNGS is Our quality management system is certified to EN-ISO 13485 by Bureau Veritas Contact CleanNA | Coenecoop 75 | 2741 PH Waddinxveen...

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CleanNGS

CleanNGS

Catalog excerpts

All CleanNa catalogs and technical brochures