qPCR Reagent Kits Speed. Confidence. Value. Sensitivity. Performance.

The Cole-Parmer detection kits utilise the sensitivity and speed of qPCR to get the most accurate data as quickly as possible. Each kit is specifically designed by our bioinformatics team to ensure the broadest possible detection profile and detection of clinically relevant strains and subtypes. Kits come with sufficient controls to reduce the chances of making the wrong call and positive copy number standard to allow for easy quantification. Each Cole-Parmer DNA reagent kit employs the same experimental setup, amplification protocol and interpretation of results. Plate setup includes: 1. Internal...

Lyophilised primer and probe mix Lyophilised master mix Lyophilised master mix resuspension buffer (liquid) Lyophilised ROX Ampule High-copy DNA for standard curve Internal extraction control DNA Primer/probe for detecting endogenous control RNAse/DNAse free water Reverse transcriptase (within the mastermix of RNA kits only) 150 tests per kit: exceptional cost per data point Available either with or without mastermix for added flexibility and work flow fit. Supplied as lyophilised components plus resuspension buffers and water (allows for ambient shipping followed by -20 storage on arrival) Positive...

The Human Pathogen section of the Cole-Parmer detection kit range can be further divided into sub categories, see below, within these sub divisions lie the most commonly requested and tested infections and diseases. Without With Mastermix Mastermix Description Sexually transmitted Infections 93940-56 93940-57 Herpes viral Infections 93942-26 93942-27 DNA, RNA, Avian Influenza A virus Subtype H7N9 RNA, All Group 1 Coronavirus genomes RNA, All Group 2 Coronavirus genomes RNA, Human Influenza Type A M2 v2.0 RNA, Human Influenza A virus Subtype H3 RNA, Human Parainfluenza virus Type 4A RNA, Human...

Without With Mastermix Mastermix Description RNA, Hepatitis A virus DNA, Hepatitis B virus v2.0 RNA, Hepatitis Delta virus RNA, Hepatitis E virus DNA, Human Papillomavirus 16 DNA, Human Papillomavirus 18 DNA, Human Papillomavirus 45 DNA, All Clostridium perfringens species DNA, Clostridium difficile (toxin B) DNA, Enteropathogenic Escherichia coli DNA, Shiga toxin (stx1) producing Escherichia coli DNA, Shiga toxin (stx2b) producing Escherichia coli DNA, All pathogenic Salmonella species DNA, All Vibrio species v2.0 DNA, All Vibrio cholerae subspecies DNA, Anaplasma phagocytophilum DNA, Borrelia...

Positive control confirms all conditions and reagents are working well. A high-concentration standard is used as positive control. Primers and probes provide highly specific, easy Internal extraction control confirms that the DNA to interpret test results with sensitivity down to extraction process was successful. Exogenous 10 copies. DNA is spiked into the lysis buffer. Endogenous control • Endogenous control confirms quality of biological sample. • Beta-actin confirms that a valid biological template was extracted. • A primer and probe mix is included to detect Beta-actin through the FAM channel....

Veterinary and Agriculture Many topical and geographically important tests have been developed to allow producers and companion animal owners/veterinarians to screen animals quickly and with the high sensitivity of qPCR. Within the veterinary and agricultural range of pathogens detection kits are diseases and infections which can have significant economic impact. Without With Mastermix Mastermix Description DNA, Acinetobacter baumannii DNA, Aspergillus fumigatus QPCR Kit, RNA, Avian Infectious Bronchitis virus (IBV) RNA, Avian Influenza A virus Subtype H7 RNA, Avian Influenza A virus Subtype...

Without With Mastermix Mastermix Description RNA, Rift Valley Fever virus DNA, Streptococcus equi subspecies equi RNA, Equine/Canine influenza (H3N8 & H7N7) RNA, Vesicular stomatitis virus DNA, All pathogenic Salmonella species DNA, Shiga toxin (stx1) producing Escherichia coli DNA, Shiga toxin (stx2b) producing Escherichia coli RNA, Porcine epidemic diarrhoea virus RNA, Vesicular stomatitis virus DNA, Bartonella henselae DNA, Babesia caballi DNA, Bordetella Bronchiseptica & Bordetella RNA, Feline Immunodficiency virus v2.0 RNA, Feline Leukemia virus DNA, Bordetella Bronchiseptica & Bordetella...

Biothreat A small list of detection kits aimed at detecting some particularly harmful viruses and bacteria such as Rift Valley Virus and E.coli 0157. DNA, Bacillus anthracis DNA, Burkholderia mallei DNA, Chlamydophila psittaci v2.0 DNA, All Clostridium perfringens species DNA, Coxiella burnetii DNA, Escherichia coli 0157:H7 v2.0 DNA, Francisella tularensis RNA, Rift Valley Fever virus DNA, Vaccinia virus DNA, C.M.Haemominutum DNA, C.M.turicensis DNA, Cryptococcus neoformans DNA, Alpha toxin producing Clostridium perfringens

Fish Speciation kits DNA, Melanogrammus aeglefinus (haddock) DNA, Gadus morhua (cod) DNA, Universal fish detection DNA, Brucella genus (All species) DNA, Campylobacter Jejuni DNA, Clostridium estertheticum DNA, All Clostridium perfringens species DNA, Coxiella burnetii RNA, Crimean-Congo Haemorrhagic Fever virus DNA, Eubacteria Bacteria Domain DNA, Mycobacterium avium subspecies DNA, All pathogenic Salmonella species Without With Mastermix Mastermix Description Meat Speciation kits 93944-64 93944-65 DNA, Gallus gallus (chicken) DNA, Anas platyrhynchos (duck) DNA, Capra hircus (goat) DNA, Melanogrammus...

QPCR-500 Real time system The QPCR-500 real time system is a high specification, economically priced real time thermal cycler that accommodates a unique 48-well polypropylene PCR plate utilising the same geometry as standard 384-well plates, but only 1/8 of the size. This enables users to dramatically reduce the qPCR reagent volumes compared to traditional 96-well instruments, saving users precious sample, whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity. A minimum volume of 5pl is validated, resulting in a more efficient...