Real Time PCR System

Introducing the Cole-Parmer® QPCR-500 Real Time PCR System, previously know as the ECO-48 The Cole-Parmer QPCR-500 Series The Cole-Parmer QPCR-500 real time PCR system is a high specification, economically priced real time thermal cycler that accommodates a unique 48-well polypropylene PCR plate utilising the same geometry as standard 384-well plates, but only 1/8 of the size. This enables users to dramatically reduce the qPCR reagent volumes compared to traditional 96well instruments, saving users precious sample, whilst still producing a strong fluorescence signal. Minimizing the plate size...

• MIQE compliant • HRM functionality is provided as standard and can discriminate class IV SNP 99.9% of the time • The QPCR-500 can utilise four colours for easy multiplexing • Industry leading ±0.1°C temperature uniformity (recorded at 95°C no settle time) • Fast cycling enables several experiments per day, all at an economical price • Fastest block-based real-time PCR system with the ability to run 40 cycles in 20 minutes (or less when optimised) • The QPCR-500 is an open platform that can utilise any chemistry, dye or PCR reagent • Calibrated for SYBR®, FAMTM, HEXTM, VICTM, ROXTM and Cy®5...

Cole-Parmer QPCR-500 thermal technology The QPCR-500 specificity and efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy, temperature must remain uniform across the entire heat block, ensuring that all samples are processed equally. The unique thermal block design of QPCR-500 achieves this with a unique heating and cooling system that provides accurate ±0.1ºC temperature control and quickly cycles from one temperature to the next. To achieve true temperature control the QPCR-500 thermal system consists of a precisely electroformed...

Optical Technology Adaptive LED Control (ALC) The QPCR-500 Real Time PCR system contains an advanced high-performance optical system that delivers precise and sensitive fluorescence detection, facilitating all 4 colour multiplex applications. The system is provided factory-calibrated for use with SYBR®, FAMTM, HEX™, VICTM, ROXTM, Cy®5 and can also be used with any qPCR fluorophores overlapping with these calibrated dyes. For excitation, two panels of 48 fixed LEDs provide excitation energy of distinct spectra, enabling broad range excitation. Each of the 48 LEDs illuminates a specific well location,...

• Absolute quantification standard curves with efficiency calculation • Relative quantification using the AACq method with support for multiple reference gene normalisation • Relative gene expression with efficiency control • Next Generation Sequencing (NGS) library quantification • Copy Number Variation: insertions, deletions, inversions • RNA Characterisation: siRNA and miRNA • Viral load • Allelic Discrimination • Microbiology: pathogen detection and identification High resolution melting (HRM) • Genotyping, methylation studies and mutation screening

• qPCR is the most precise method for quantifying libraries prior to cluster generation • Works at concentrations below the detection threshold of conventional spectrophotometric methods • Validation of array and sequencing quality data • Academic, Government and Corporate laboratories • Human research, AgriGenomics, Translational, Forensics and many more markets • Probe, DNA Binding Dye and HRM chemistries • Gene expression, Viral detection, Sequencing Library • Preparation, Genotyping and Mutation Screening applications

Rapid cycling The QPCR-500 has rapid heating and cooling rates. Average ramp rates of 5.5°C/sec reduce overall PCR run times. The typical run time for a 40 cycle PCR protocol is under 40 minutes. In testing, the quickest optimised programme enabled a 40 cycle qPCR experiment to complete in just 15 minutes. High throughput QPCR-500 thermal control is superior to traditional 96-well peltier systems where thermal efficiency decreases as distance from the peltier increases. With QPCR-500, faster and more precise cycling increases sample throughput because the need for multiple replicates is reduced...

The QPCR-500 system comes with two distinct software elements. The first, Control, drives the units set up and experimental runs while Study is the analysis package. The QPCR-500 software are both open licence, you can install them on as many PCs as you like, and are MIQE compliant. • User-installable, intuitive, easy to use software that integrates user control, realtime data collection, and advanced data analysis • Supplied fully featured including HRM capability • New software version 5 enables a widened chemistry library and contains new thermal profiles • Facilitates rapid thermal cycling:...

The ergonomic plate loading dock allow for easy loading of the plate and is backlit to allow the user to easily visualise which wells have been loaded. • Base with LED backlight • Plate adapter fits standard centrifuge rotors • Includes plate sealing tool • Control and Study software USB stick • Plate seals (Pack of 50) • Power and Ethernet Cables

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