Corning® PureCoat™ ECM Mimetic Cultureware
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Corning® PureCoat™ ECM Mimetic Cultureware Natural Performance. Scientifically Defined.

The First Animal-Free Synthetic Surface To Mimic Natural ECM Attachment & Functionality. Next generation Corning® PureCoat™ ECM Mimetic Cultureware is coated with biologically active synthetic animal-free peptides that are covalently linked to a proprietary surface to provide a highly consistent alternative to complex extracellular peptides. The peptides are rationally designed to mimic the cell attachment motifs of native ECM proteins, which promotes optimal cell binding and signaling in a broad range of serum-free, xeno-free, and animal-free media formulations. There are two Corning...

Enables a Fully Defined Cell Culture Environment. Corning® PureCoat™ ECM Mimetic cultureware has been demonstrated to function in a broad range of cell types and defined serum-free, xeno-free, and animal-free media. The cultureware enables researchers to tightly and reproducibly control the cell culture environment for predictable cell expansion and differentiation outcomes. Consistency Built on cGMP-Compliant Manufacturing and Animal-Free Traceability. Corning PureCoat ECM Mimetic cultureware is manufactured in a cGMP compliant facility which meets ISO 9001:2008 and 13485:2012 standards...

Human Mesenchymal Stem Cells (hMSCs) Ease of isolation and expansion of hMSCs makes them an attractive tool for allogenic transplant in regenerative medicine and tissue engineering applications. Typical ex vivo expansion of hMSCs requires either bovine serum-containing media or a defined and serum-free media, plus a coating of human or animal-derived extracellular matrix (ECM) protein. Corning® PureCoat™ ECM Mimetic Cultureware Fibronectin peptide functions as a synthetic, animal-free replacement for natural human-derived biological coatings for expanding and differentiating hMSCs in...

Comparable Multilineage Differentiation Potential. An intrinsic property of mesenchymal stem cells is their ability to differentiate into various somatic tissues (Adipocytes and Osteocytes). hMSCs cultured on the Corning® PureCoat™ ECM Mimetic Fibronectin peptide reliably retain their multipotency after multiple passages, comparable to the human-derived ECM coating proteins. Differentiation to Adipogenic Lineage After 3 Passages Oil Red O stain qualitative analysis. Corning PureCoat ECM Mimetic Fibronectin Peptide MSC Expansion Kit XF Protein Coated Surface Uninduced Control Differentiation...

Human Keratinocytes Keratinocyte accessibility, proliferation potential, and ease of culture has enabled the use of these cells in regenerative medicine applications. Typical ex vivo expansion of Keratinocytes requires either coating of the culture vessel with human or animal-derived extracellular matrix protein or a growth medium with bovine serum or animal-derived components. Corning® PureCoat™ ECM Mimetic Cultureware Collagen I peptide functions as a synthetic, animal-free replacement for natural human- or animal-derived extracellular matrix coatings for expanding human Keratinocytes in...

Comparable Cell Functionality. Human neonatal Keratinocytes cultured on the Corning® PureCoat™ ECM Mimetic Collagen I surface demonstrated comparable cell migration potential compared to cells cultured on rat tail Collagen I and recombinant human Collagen I. Cell functionality/migration assay A ‘wound’ or scratch was introduced in the cell monolayer. Images captured the cell migration at 0, 2, 3, and 4 hour time points

Endothelial Progenitor Cells (EPCs) Endothelial colony forming cells (ECFCs®) have potential uses in regenerative medicine, such as cell therapy for cardiovascular disease, or use as biomarkers to assess disease risk. Expansion of these cells requires coating of a culture surface with human or animal-derived extracellular matrix (ECM) proteins. Corning® PureCoat™ ECM Mimetic cultureware functions as a replacement for human- or animal-derived ECM proteins in serum-free or low-serum environments. Comparable Cell Growth and Morphology. Human ECFCs cultured on Corning PureCoat ECM Mimetic...

Reliable Cell Functionality. Human ECFCs® cultured on Corning® PureCoat™ ECM Mimetic cultureware surfaces retain normal cell functionality as demonstrated by angiogenesis capillary tube formation assay and LDL uptake assay. Tube formation assay. ECFCs expanded on Corning PureCoat ECM Mimetic Fibronectin and Collagen I Peptide surfaces were plated on Corning Matrigel® matrix and allowed to undergo capillary tube formation overnight. Fibronectin peptide LDL uptake assay. ECFCs expanded on Corning PureCoat ECM Mimetic Fibronectin and Collagen I Peptide surfaces demonstrated the ability to...

Other Attachment-dependant Mammalian Cell Types Corning® PureCoat™ ECM Mimetic Fibronectin and Collagen I peptide surfaces support enhanced attachment and proliferation of Vero and CHO-K1 cells in serum-free and protein-free media, respectively, over tissue culture (TC)-treated surfaces. Enhanced Attachment and Proliferation of Vero Cells. Vero cell attachment and growth were increased on Corning PureCoat Fibronectin and Collagen I peptide surfaces, compared to the uncoated TC-treated surface (recommended for Vero cell culture with the serum-free, low protein growth medium* used). In...

Enhanced Attachment and Proliferation of CHO-K1 Cells. CHO-K1 cells cultured in a protein-free, chemically defined medium* on Corning® PureCoat™ Fibronectin peptide surface grew faster than those on the TC-treated surface, as reflected by their confluence. Consistent with published evidence on the growth of CHO-K1 on natural Collagen, Corning PureCoat Collagen I peptide surface did not support CHO-K1 attachment and growth. CHO-K1 cells cultured on the Corning PureCoat ECM Mimetic Collagen I peptide CHO-K1 cells cultured on the Corning PureCoat ECM Mimetic Fibronectin peptide CHO-K1 cells...