LE220Rsc Focused-ultrasonicator High-performance, Scalable, Automated Sample Preparation As the highest power and fastest sample processing system in the Covaris portfolio, the LE220Rsc rapidly delivers AFA-energetics ® to standard SBS format plates using the scanning mode. It offers tunable acoustic energy, integration with robotic platforms, and more power than the LE220-plus and R230 Focused-ultrasonicators. This instrument enables a variety of novel high power, fast process applications such as lysis of difficult to lyse microorganisms (for example, yeast) in a 384 well plate and dissolution of difficult to solubilize compounds. Feature Accurate, reproducible, and robust sample preparation Automated workflows with full robotic integration Multiple sample types Multiple isothermal energy delivery modes Tuneable, non-contact processing Compatible with most processes Broad temperature range at high power Simplify highly complex workflows Multiple applications: nanoparticle formulation, microbe lysis, and hydrophobic compound dissolution Supported Applications: • DNA/Chromatin shearing • Cell Lysis: mammalian, bacterial, yeast • Biomolecule extraction: FFPE, tissue, and whole blood • Sample processing: bead mixing • Compound screening Fully Automated NGS Library Preparation Workflow The LE220Rsc Focused-ultrasonicator aids in the movement of samples through numerous steps in a workflow on multiple instruments without human intervention. Processing time for a 96 and 384 plate ranges from 5 to 16 minutes, depending on desired fragment size. DNA purification and normalization on a liquid handler ROBOTIC ARM MOVES PLATE ROBOTIC ARM MOVES PLATE Library preparation on a liquid handler API Integration LE220Rsc Focused-ultrasonicator AFA-TUBE - 384 Scanning LE220Rsc520249 96 AFA-TUBE TPX Plate -3.2 offset - NGS The Covaris API integration toolkit contains everything needed to write a driver for SonoLab 8.4. This includes: • Emulation user interface showing all the remote commands • Example code for scripting each command • Cut and paste capability for ease of implementation

Extract HMW DNA from Microbial Samples Product Specifications Reproducible and robust data for cell lysis and HMW DNA extraction from difficult-to-lyse organisms including yeast and bacteria. (Figure 1 and 2) Size (bp) Figure 2. Superposition of electropherograms (AATI - Fragment Analyzer DNF-492-33 - SS Large Fragment) showing an extremely narrow DNA fragment distribution directly extracted from a primary E. coli (~ 4 x 108 cells, buffer volume = 30 pLJ sample without purification. In scanning mode, processing time less than 1 minute for 96 samples. Part Number Product Name Description High-performance,...