Cytek®Aurora
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Cytek® Aurora Say Hello to a New Reality Meet Aurora: A prodigy incorporating a unique combination of innovative technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora system suits every laboratory’s needs, from simple to high-complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art...

Cytek® Aurora Say Hello to a New Reality Aurora’s Revolutionary Technologies: From Vision to Reality The Aurora system is capable of up to 67 detection channels (64 fluorescence channels, FSC, blue laser SSC, and violet laser SSC) and is empowered by revolutionary technologies, including: Proprietary high sensitivity Coarse Wavelength Division Multiplexing (CWDM) semiconductor detector arrays, enabling more efficient spectrum capture for dyes emitting in the 365-829 nm range. High bandwidth electronics design scalable up to 67 channels. Robust vacuum fluidics system enables ultimate...

Cytek® Aurora Say Hello to a New Reality Resolving Challenging Dye Combinations The detection of some fluorochrome combinations by conventional flow cytometry presents a challenge due to high amounts of spectral overlap (Figure 1, 4). The Aurora system addresses this challenge by using differences in full emission spectra signatures across all lasers to clearly resolve these combinations, even if the populations are co-expressed (Figures 2, 3, 5, and 6). Example 1: APC and Alexa Fluor 647 Gated on lymph CD3+ % Parent: 17.43 Figure 1: Spectrum plots from a conventional spectrum viewer shows...

Cytek® Aurora Say Hello to a New Reality See More Clearly with Autofluorescence Extraction The Aurora system’s implementation of full spectrum cytometry enables the use of autofluorescence extraction to further improve data clarity. Certain sample types, such as yeast and tumor samples, present the challenge of high autofluorescence. For these challenging applications involving highly autofluorescent particles, let the software’s autofluorescence extraction tool bring new levels of resolution. Example 1: PrimeFlow™ RNA Assay Human U937 cells were subjected to the PrimeFlow™ RNA Assay. The...

Cytek® Aurora Say Hello to a New Reality Small Particles in Full View With its onboard 100 mW 405 nm laser and highly sensitive violet SSC detector, particles nearing 100 nm in size can be analyzed. The Aurora system opens the door to a wide variety of small particle applications, taking what was once hidden and placing it in full view. Resolution of ApogeeMix from Apogee Flow Systems (www.apogeeflow.com), a mixture of silica and polystyrene (PS) beads ranging from 110 nm to 1300 nm, when acquired on the Aurora system. The smallest particles can be easily identified above background. Data...

Cytek® Aurora Say Hello to a New Reality More Choice, Greater Flexibility, Easier Setup The optical design combined with the unmixing capability in SpectroFlo® software allows greater fluorochrome choice, panel flexibility, and easy setup without having to change filters. The three-laser configuration provides outstanding multi-parametric data for a wide array of applications. Markers and fluorochromes in a 24-color panel designed for identification of circulating cell subsets in human peripheral blood are summarized in the table below: 24-COLOR DATA On the next page, this 24-color panel is...

3 Lasers, 24 Colors, Unparalleled Resolution Lineage Negative Cells Double Negative Cells 47215 Lakeview Blvd Fremont, CA 94538

Aurora Makes It Possible CD8+ Effector Memory Cells CD4+ Effector Memory Cells 47215 Lakeview Blvd, Fremont, CA 94538 Treg Cells

Cytek® Aurora Say Hello to a New Reality See More with the UV Laser With the addition of the UV laser and a total of 64 fluorescence detectors, the Aurora system now has the power to take highly-multiplexed assays beyond 30 colors. Incorporation of the UV laser takes the Aurora platform to the next level. 35-Color Panel Markers and fluorochromes in a 35-color panel are summarized in the table below. Human peripheral blood mononuclear cells were stained, washed, and acquired on a five-laser Aurora system. Yellow Green Pacific Orange Dead Cells LIVE/DEAD™ Blue LIVE/DEAD™ is a trademark Thermo...

Cytek® Aurora Say Hello to a New Reality Double Negatives T Cells 47215 Lakeview Blvd, Fremont, CA 94538

Cytek® Aurora Say Hello to a New Reality SpectroFlo® Software Guided Workflows The SpectroFlo software offers an intuitive workflow from quality control (QC) to data analysis with technology-enabling tools that simplify running any application. QC and Setup: Extra Tools: Run Daily QC to monitor instrument performance and add reference controls. Unmix data using controls from different experiments or apply virtual filters to your data. Users: For administrative controls. Add or remove experiment templates, worksheet templates, fluorochrome information, QC bead information, and more....

Cytek® Aurora Say Hello to a New Reality Get to Know Our New Automated Sample Loader (ASL) Meet the ASL The ASL offers more versatility when running your samples at high-throughput. In addition to acquisition from 96-well plates, the ASL is compatible with 96-deep well plates and 40-tube racks. For each carrier type, Cytek has provided preset mixing speeds and frequencies, which are also fully customizable to meet your individual experimental requirements. The ASL is designed to streamline experimental workflows and integrates seamlessly into the Aurora system. Reliable and flexible...

Cytek® Aurora Say Hello to a New Reality We Are Here to Support You Cytek Biosciences is dedicated to enhancing our customers’ user experience. The Aurora system is backed by our worldclass service and support team that can provide phone or field-based assistance. Various levels of maintenance options are available to meet your needs now, and in the future. Technical Support We have a worldwide team of field service engineers and technical application specialists at your service. To maintain your instrument and keep it running well, you can choose the right service contract for your needs....