Streamline your single-bacteria and single-yeast isolation and cloning

Streamline your single-bacteria and single-yeast isolation and cloning Microbiology is no stranger to innovation, and with current technological advancements, an increasing need for isolating and cloning bacteria and yeast has catapulted the field to new heights. The acceleration of single-cell isolation and cultivation of unknown species from complex microbiome samples is sparking a revolution in microbiome research, and our evolving knowledge of the microbiota is creating opportunities to engineer new diagnostic techniques that benefit many fields from ecology and agriculture to medicine, forensics...

Reducing the risk of cross-contamination The B.SIGHT uses our new EASY.ON cartridges, which have been precisely engineered by microfluidic experts to ensure cell viability with the gentlest handling, even with the minimum 5 µL volume. The ability to dispose of the cartridges eliminates the risk of cross contamination between samples. Plus, setting up your experiments has never been easier as the EASY.ON cartridge is magnetically mounted for quick and easy loading. Precise single cell dispensing into PCR plates Equipped with an AOC system, the B.SIGHT can circumvent the need for manual droplet...

Greater insights from faster single bacteria isolation The B.SIGHT combines our patented single-cell dispensing technology with an intuitive and fast software. Isolation of single bacteria into multiwell plates can be done in less than 3 minutes. Separate high-resolution brightfield and fluorescence images are obtained together with an overlay image. The instrument’s operating software analyzes cell morphology to isolate single cells according to set parameters such as size, roundness and even fluorescence intensity, which then can be integrated with subsequent NGS Data. Microbiome research Unprecedented...

Fully Enclosed Anaerobic Cell Isolation The B.SIGHT has successfully and reliably isolated even the most sensitive samples, such as obligate anaerobes organisms. Thanks to its small footprint as well as low particle and heat emissions, the B.SIGHT can operate within an anaerobic chamber, thus opening the door for a simpler and more convenient isolation process. The B.SIGHT can also be fully controlled by a remote terminal outside the chamber. Automation The B.SIGHT is tailored for automated workflows; its lid can be programmed to open and close automatically for a seamless integration with a...

Technical Specifications Samples Uncultured microbiota, cultured E.coli, yeast, etc. Cell size Cell morphology or fluorescence Droplet volume 96,384-well plates, PCR or single well for agar Compatible plate height Up to 27 mm (ex: low-profile deep-well plates) Automated Offset Correction (AOC) for precise Bottom imaging using 5x objective lens positioning of the single cells Optical resolution 2.2 µm Camera CMOS Nozzle imaging Magnification 20x Dual-channel: Brightfield and fluorescence Fluorescence imaging Processing times Cartridge Ex. 488 nm Em. 504 - 596 nm Single-cell dispensing 96-well...

We create the future of health. CYTENA, A BICO COMPANY CYTENA is a leading provider of high-precision instruments for isolating, dispensing, imaging, analyzing and handling biological cells. The company continues to build on the success of the single-cell dispensing technology it patented as a spin-off from the University of Freiburg, Germany, in 2014. Today, as part of BICO, the world’s leading bio convergence company, CYTENA’s award-winning devices are still manufactured in Germany and used at prestigious academic and pharmaceutical labs around the world to automate workflows in numerous application...