CytoSMART Lux3 FL fluorescence imaging
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Automated fluorescence imaging inside your incubator CytoSMART™ Lux3 FL

Improve your research with fluorescence live cell imaging Using fluorescence live-cell imaging, researchers can get more relevant data about their experiments. The CytoSMART Lux3 FL is a small fluorescence live-cell imager equipped with one brightfield and two fluorescent channels (green and red). The device enables researchers to unravel cellular processes in real-time, providing lots of valuable data about the experiments. The CytoSMART™ Lu3 FL: + Integrated image analysis of brightfield and fluorescence area or fluorescence object count. Time lapse movies to investigate the development...

Applications The use of fluorescence live-cell imaging has become increasingly valuable in the fields of drug discovery, tissue engineering, immunology, immunotherapy, and cancer research. With the CytoSMART™ Lux3 FL you have a big advantage over your colleagues and competitors. With our cloud-based solution, you have access to the following application anywhere and anytime you need it: + Cell viability (Fig. 2) + Cellular processes (Fig. 3) + Hypoxia / oxidative stress markers (Fig. 5) + Transfection (Fig. 6) + Co-culture + Nanoparticles + and more However, you are not limited to these...

Live insight in cellular processes Simultaneous analysis of multiple cell features The CytoSMART Lux3 fluorescence live-cell microscope automatically creates time-lapse movies that contain many cellular features. The videos are made from inside the incubator without disturbing your cells and can be immediately accessed and analyzed remotely via the CytoSMART Cloud, providing real-time updates on your cell cultures and running experiments. Using CytoSMART Lux3 FL, you can analyze green and red fluorescent tags simultaneously with your brightfield images. This not only saves you time but also...

Figure 7. The open design of the CytoSMART™ Lux3 FL enables easy monitoring of cells cultured inside the incubator. Figure 5. Proliferating cells in 3D tumour spheroid indicated Figure 6. Overlay of green fluorescent and brightfield images of by green fluorescence. Red fluorescence represents the 3T3 cells transfected with CellLight Nucleus-GFP, BacMam 2.0. drug infused in tumour spheroid. The drug uptake and effect Images were taken 18 h after the start of the transfection process. on proliferation of the tumour cells are followed using the CytoSMART™ Lux3 FL. Image courtesy of Catarina...

Easy data storage and image analysis The CytoSMART™ Lux3 FL can be set to record images at specific intervals (between 5 min - 12 h.) for minutes, hours and days. In fact, it is one of the few systems that can run for weeks. The recorded images are sent to the CytoSMART™ Cloud where they are analyzed using our custom, cloud-based, image analysis software. You can select the appropriate image analysis algorithm, such as confluence detection, according to the experiment you are performing. in almost real-time from anywhere on any pc, laptop, tablet or mobile phone with internet access. All...

Plates, dishes, flasks or microfluidic chips The CytoSMART™ Lux3 FL can image cells cultured in a wide range of culture vessels including T-flasks, petri dishes, well plates, culture slides and microfluidic chips. You can simply conduct an experiment in any culture vessel or microfluidic chip. Place the CytoSMART™ Lux3 FL device in the incubator. Connect the device’s power cable and the USB3 cable to your PC or laptop. Start the PC. Download the CytoSMART Lux3 FL application. Open the app and you are set to go! Figure 8. The CytoSMART Lux3 FL allows monitoring of a wide range of different...

Frequently Asked Questions Q: What is the CytoSMART Lux3 FL? A: The CytoSMART Lux3 FL is a small fluorescence live-cell imaging microscope equipped with one brightfield and two fluorescent channels (green and red). The device allows users to track dynamic cellular processes with high specificity by taking high-quality fluorescence images to create real-time time-lapse movies. Experiments’ data can be accessed and analyzed remotely via the CytoSMART Cloud portal. Q: For what applications can I use the CytoSMART Lux3 FL? A: The Lux3 FL can image and analyze various applications, for example,...

Channels Brightfield, green and red fluorescence channels Ordering information Magnification 10x fixed objective, digital zoom to 20x at # Product Quantity Fluorescence filters Green: Excitation: 452/45 nm, Emission: 512/23 nm MAB-1008 CytoSMART™ Lux3 FL 1 Red: Excitation: 561/14 nm, Emission: 630/90 nm Camera 6.4 mp cmos Request quote on www.cytosmart.com Data formats JPG, XLSX, MP4 Image size 2072 x 2072 pixels Culture vessels well-plates, petri dishes, flasks, microfluidic chips and custom culture vessels Computer requirements Windows 10 Operating conditions 5-40 °C, 20-95% humidity...

About CytoSMART CytoSMART Technologies is a specialist in development and manufacting of smart microscope systems for life science labs. The company was founded in 2012 by a team of biologists and engineers who were convinced that a new generation of miniaturized microscopes, powered by artificial intelligence for image analysis, would allow biologists to make discoveries more efficiently and at a larger scale. In 2018 CytoSMART was selected by Microsoft for its prestigious Scale Up program. CytoSMART’s microscopy solutions are used in over a thousand laboratories around the world....

CytoSMART™ Technologies B.V. Emmasingel 33 5611 AZ Eindhoven The Netherlands Website: www.cytosmart.com Email: info@cytosmart.com For Research Use Only. Not to use in diagnostics procedures. ©2020 CytoSMART™ Technologies B.V., the CytoSMART™ logo is a trademark and/or registered trademark of CytoSMART™ Technologies B.V.