CytoSMART Omni: Label Free Assay - Colony Formation
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CytoSMART Omni: Label Free Assay - Colony Formation - 1

CytoSMART Omni live-cell imaging Time-lapse brightfield imaging to study cell health, viability, colony formation and migration Users can perform kinetic assays that capture cellular behavior for days or even weeks at a time. cell level detail full plate overview Automated colony detection: HEP-G2 cells with colony mask overlay. The image detection algorithm filters colonies based on user specifications on size, circularity and perimeter and follows their growth over time. Cells were recorded for 75 hours with a 3 hour time interval. 0,8 Colony formation assays provide insight into 6E+03 5E+03 0,6 the clonogenicity of cells. The formation and 4E+03 0,4 growth of colonies can be monitored using 3E+03 2E+03 0,2 the CytoSMART Omni. Bright-field images 1E+03 0E+00 are automatically generated from within 0,0 cell 0 0 20 40 60 80 culture incubators over periods of days. Whole�me [hours] well surfaces are captured to get a full sample 9E+04 6E+03 overview. 8E+04 stdv colony size [μm²] Colony count Integrated image analysis algorithms in the 3E+03 CytoSMART Cloud provide information on the 2E+03 1E+03 number of colonies, colony circularity and size. 0E+00 The highlighted orange areas in the image 0 0 20 40 60 80 indicate the colonies that are used for the �me [hours] analysis. Both the numerical data and images can easily be extracted after analysis.* 4E+03 https://www.cytosmart.com/products/omni *Research use only. Not intended for diagnostic purposes

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