1. Introduction The BioTherm Dry Ice systems are qualified as a range that holds a payload temperature of -20°C or lower for a minimum of 72 hours. It would like to be understood the duration the BioTherm Dry Ice systems are qualified to hold a payload temperature of -90°C to -60°C, what mass of dry ice is required and what payload volume is left available. DGP Intelsius BioTherm Dry Ice Qualification Reports testing data encompasses data retrieved up until the internal payload reaches 0°C, the state in which all dry ice has dissipated. Due to this method of testing, BioTherm Dry Ice systems can be reliably used for payloads requiring any temperature limits between -80°C and 0°C. DGP Intelsius qualification reports, as specified in section 2, include an out-ofspecification line at -20°C, a standard payload temperature used throughout the packaging industry. Current qualification reports can be used as evidence of alternative – 60°C payload temperature requirements, including packing instructions, graphs and raw data. For clarity, this supplementary document has been published to summarise the durations of the BioTherm 7, 15, 30, 45 & 80 for a -60°C payload temperature.

The BioTherm systems were qualified against the ISTA 7D Summer temperature profile. The duration the systems achieved at keeping a payload temperature of -90°C to -60°C, the mass of dry ice used, and the dry ice loss rate can be found in the table below. The testing data is displayed in the graphs.

BioTherm 10 Dry Ice vs ISTA 7D Summer BioTherm 15 Dry Ice vs ISTA 7D Summer

BioTherm 45 Dry Ice vs ISTA 7D Summer Kelly Roy 29th August 2024 P23087-J001-R1.3 Page 4 of 7

BioTherm 80 Dry Ice vs ISTA 7D Summer

3.1 From the testing data it can be concluded that the BioTherm Dry Ice system sizes 30, 45 and 80 would be able to achieve a duration of 100+ hours at -90°C to -60°C. This is not a possible option for the smaller BioTherm systems due to the internal volume being at maximum capacity. However, the smaller BioTherm systems 7, 10 and 15 can be used successfully as a 60+ hour system. 3.2 The dry ice needed for each system and payload volume remaining can be seen in the table below. 3.3 Raw data results qualifying the BioTherm 7, 10, 15, 30, 45 and 80 durations to -60°C can be found in Qualification...

The BioTherm 45 can achieve a payload temperature of -60°C for 103 hours with 28.4kg or 62.61lbs dry ice, leaving a 16.8L payload volume. The BioTherm 80 can achieve a payload temperature of -60°C for 120 hours with 43.5kg or 95.9lbs dry ice, leaving a 39.0L payload volume. Report Produced By: Kelly Roy Signed __________________________________________________________ Date: 29 Aug 2024 Position Research and Development Scientist