DNA Methylation Solutions
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SOLUTIONS TO STUDY DNA METHYLATION TARGETED AND GENOME-WIDE Optimal for NGS, Sanger sequencing, Pyrosequencing & qPCR

Understanding DNA Methylation DNA methylation plays key roles in numerous biological processes such as genomic imprinting, control of transcription, embryonic development, stem cell reprogramming and carcinogenesis. In mammals, DNA methylation occurs primarily as 5-methylcytosine (5-mC), which is usually found at CpG dinucleotides. An additional mark, 5-hydroxymethylcytosine (5-hmC), has been implicated with an alternative role as a regulator of gene expression and cellular differentiation as well as an intermediary in the active demethylation process. Study 5-mC and 5-hmC Sodium bisulfite...

Find your DNA methylation analysis solution Target Single nucleotide Methylation Genome-wide Innovating Epigenetics Solutions

according to your needs 25x106 CpGs coverage Generate bisulfite-converted libraries ready for single or paired-end sequencing of the whole genome C02030034 Generate bisulfite-converted libraries ready for single and paired-end sequencing of CpG-rich parts of the genome C02030032 Bisulfite convert gDNA ready for regionspecific analysis like (q)PCR, Sanger/ pyrosequencing (SP) C02030030 MagMeDIP-seq Package From gDNA extraction to library preparation - prepare ready-to-sequence libraries of immunoprecipitated methylated DNA C02010040 Prepare libraries of captured methylated DNA combining MBD...

MagMeDIP qPCR/hMeDIP or MagMeDIP-seq Lyse cells and extract DNA Perform DNA immunoprecipitation (-sequencing) to estimate the DNA modification status of your sample using the 5-methylcytosine antibody (5-mC) or 5-hydroxymethylcytosine antibody (5-hmC). Prepare library for MeDIP-seq Immunoprecipitate, wash and isolate DNA Innovating Epigenetics Solutions

AUTO EQUIPMENT NEEDS WHICH KIT FOR WHICH STEP? (U

Your Full MeDIP-seq Solution The MagMeDIP-seq Package, our complete solution, includes MagMeDIP qPCR, IPure, iDeal Library prep and an optimized MeDIP-seq protocol to offer the highest accuracy, reliability and performance. Library prep • Start-to-finish : Complete kits include DNA extraction module, highly validated antibody 33D3, spike-in controls and qPCR primer pairs for control regions • Easy to use with user-friendly magnetic beads and racks • Validated protocols provide reproducible and consistent data - using as low as 100 ng input DNA • Highest quality purification with IPure •...

High specificity of the MagMeDIP-seq Package. The coverage profiles on the control regions of one human blood sample show excellent specificity of results. (A) No methylation detected in the negative control region. (B) High methylation detected in the positive control region. The perfect combination for best-in-class results: magnetic IP technology with ultra-validated antibodies Diagenode emphasizes strict quality standards and actual validation for all of our antibodies. Our partnerships with the leading epigenetics experts and epigenetics consortiums have allowed us to validate our...

Bisulfite conversion Bisulfite conversion reagent chemically modifies non-methylated cytosines (C) into uracil (U) while methylated cytosines (5-mC) remain unchanged. For single locus analysis, the region of interest is amplified with PCR which can be followed by Sanger sequencing or pyrosequencing. Convert DNA • Rapid bisulfite conversion of DNA - only 1 hour reaction time • Simple workflow, 3 steps • High-yields of converted DNA for methylation analysis Desulfonate, spin, wash, elute Bisulfite conversion Converted DNA ready for methylation analysis Sequencing applications Methylation levels...

Whole Genome Bisulfite Sequencing (WGBS) Whole Genome Bisulfite Sequencing (WGBS) is a single nucleotide resolution technique that allows the user to study DNA methylation sites and their role in gene regulation. Diagenode’s Premium WGBS Kit is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina® platforms. Diagenode’s Premium WGBS Kit contains specially designed enzymes and buffers needed for genome-wide bisulfite sequencing. • Wide range of DNA inputs: 5 ng - 1 µg. • Adaptor sets (6-12-24) available separately for multiplexing •...

Reduced Representation Bisulfite Sequencing (RRBS) Methylation typically targets cytosine in a CpG context in vertebrates. In RRBS, by using the restriction enzyme MspI (CCGG target sites) followed by size selection, DNA is enriched to represent CpG-rich regions (including CpG islands). Thus, RRBS is a powerful and cost-effective method to efficiently analyze DNA methylation at the single nucleotide level at approximately 20x lower cost compared to whole genome bisulfite sequencing (WGBS). • Excellent coverage – 4 million CpGs • Confidence in results – Positive and negative spike-in...

COMPATIBLE WITH ALL VERTEBRATES Discover anything Diagenode RRBS SIP – Software for Intelligent Pooling Pooling techniques allow you to manipulate fewer tubes, providing an easier-to-handle and cost-effective protocol. 2.  Library preparation 3.  Size selection 5.  Library amplification 6.  Clean up Diagenode's new online intelligent pooling aid provides the optimal pool design for RRBS to meet your specific sample and analysis needs: • Time-saving – Avoid complex calculations • ighest pooling efficiency based on qPCR H quantification – Bring the power of pooling to the highest • owerful –...

A quick glance: DNA methylation kit features Bisulfite conversion Premium Bisulfite Downstream application Sanger, Pyroseq, (q)PCR Initial fragment size (bp) Turnaround time Restriction enzyme All library prep reagents Automated Kits Manual Kits Innovating Epigenet

KITS KITS iDeal Library prep iDeal Library prep MagMeDIP-seq hMeDIP-seq

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