Innovating Epigenetic Solutions High resolution ChIP-seq and MeDIP-seq profiles Automated library preparation for Next-Generation Sequencing Reduces hands on time to just 30 minutes Reduces variability between operators and labs Ideal for low sample starting amounts

DIAGENODE AUTOMATED SOLUTIONS FOR EPIGENETICS Standardize and streamline your epigenetic research Diagenode IP-Star® Compact automates immunoprecipitation assays with increased reproducibility Diagenode, the leading provider of complete solutions for epigenetic research, offers a variety of end-to-end systems to streamline DNA methylation and chromatin immunoprecipitation workflows. Central to this full offering is Diagenode’s IP-Star® Compact,a simple yet robust automated bench-top instrument that standardizes different epigenetic applications (i.e. ChIP, MeDIP or MethylCap). Diagenode designed...

Walk-away automated system •  Magnetic-bead technology for excellent reproducibility •  Open platform allows changes in protocol parameters •  Simple touch screen interface •  Minimal footprint •  Processing up to 16 samples •  Protocols optimal for 100 µl or 200 µl volumes •  Automated reagent dispensing •  Controlled temperatures via Peltier blocks Overview of the Automated System Reagent container module Reagent container 96 well plate module 96 well plate module module Dustbin The Magtration® Technology Diagenode’s Automated System uses the principle of bead-based magnetic separation. Magnetic...

DIAGENODE AUTOMATED SOLUTIONS FOR EPIGENETICS Automated Protocols • Chromatin immunoprecipitation (ChIP) • Methylated DNA Immunoprecipitation (MeDIP) • Hydroxymethylated DNA Immunoprecipitation (hMeDIP) • Methyl-binding domain protein capture assays (MethylCap) • Sequential ChIP (Re-ChIP) • Bisulfite conversion • RNA immunoprecipitation (RNA-IP) • DNA purification (IPure) • Library preparation for NGS platforms Successful implementation of automated ChIP-seq experiments on the IP-Star® Compact Ilf >> ChIP Process Assay Analysis Data Analysis Auto ChIP-seq assays were performed on the IP-Star®...

ChIP kits Auto True MicroChIP kit Auto iDeal ChIP-seq kit for Histones • Compatible with 5,000-100,000 cells per IP • Validated for histone antibodies • Ideal for whole genome sequencing from low amount of chromatin when combined to the MicroPlex Library Prep kit Library generation with the MicroPlex kit. ChIP assays were performed on 10,000 and 100,000 HeLa cells with Diagenode H3K4me3 antibody (0.25 pg/reaction). Libraries were made with the MicroPlex Library Preparation Kit. The generated libraries were then analyzed on an Illumina® HiSeq2000. Cluster generation and sequencing were performed...

DIAGENODE AUTOMATED SOLUTIONS FOR EPIGENETICS • Compatible with 50 ng to 1 pg per IP • Validated using Diagenode 5-mC monoclonal antibody 33D3 - Premium (Cat. No. MAb-081-100) Optimal for genome-wide screening of methylated CpGs Automated MeDIP with the IP Star Manual MeDIP Automated profile was confirmed to • Compatible with 1 pg per reaction • Validated using Diagenode's fusion MethylCap protein • Allows fractionation for methylated DNA based on density of methylated CpG islands • Ideal for any MBD-seq and MBD-qPCR experiments high 30 -medium 30 -low CpGs 1 -CpG islands Methylation patterns...

NGS Library Preparation kits MicroPlex Library Preparation™ kit v2 • 3 times faster than TruSeq™ - only 2 hours • 48 samples processed per run • Picogram inputs (50 pg) • High sensitivity ChIP-seq - low PCR duplication rate • Great multiplexing flexibility with 48 barcodes (8 nt) included iDeal Library Adaptor dsDNA template Stem-loop Adaptor Cleavable Replication Stop Nick Blunt-end Ligation ^fapto!^ 4^7 High Fidelity Amplification Figure 1. An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina® NGS platforms using a fast and simple 3-step protocol....

The ability to produce libraries starting with low input DNA is primordial when using ChIP-seq. I am a ChIP-seq user and I always use the MicroPlex kit for library making from my immunoprecipitated DNA. Not only it's quick and requires as little as 50 pg of input DNA but it also never fails. I've made over 100 libraries using the MicroPlex library preparation kit, and none of them failed. I use the Microplex kit for preparing libraries from immunoprecipitated DNA and from genomic DNA with low concentration. As part of a genomics lab, I regularly prepare libraries from a large number of samples....