Diagon reagent kits
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Solutions beyond imagination

Testing laboratories worldwide count on the best solutions for fast and reliable testing of differ-

Diagon PCR kits are applied in laboratories for the quantitative and qualitative detection of genomic nucleic acids of microbes contaminating samples from diverse source (water/liquid and food/solid samples, clinical specimens). Microbial nucleic acids are analysed by real-time PCR amplification. Our primer-probe pairs are dedicated to unique sequences encoding both for pathogenity markers, typical bacterial enzymes and taxonomically conserved regions (16s RNA coding genes, etc.). This amplification system demonstrates the selected genes together with their regulatory elements in one DIAGON...

Bacterial Cell Wash Solution DiaExtract Bacterial Cell Wash Solution (DiaExtract BCWS) is a proprietary buffer system for the removal of non-viable pathogens from diverse cell suspen- The method is based on structural and functional differences between viable and non-viable microbial cell surfaces and separates the two cell populations according to the chemical composition of the buffer. This results in the formation of two distinct phases easy to separate by centrifugation. After several washing steps the final sedimentation provides the pellet containing the viable cell fraction ready for...

DiaExtract Microbial DiaExtract microbial DNA isolation kit is applied in laboratories for the extraction of microbial double stranded DNAs from a wide variety of liquid and solid specimens of animal and human source. This proprietary DNA extraction method involves mechanical destruction and/or enzymatic lysis resulting in homogenisation of samples followed by selective extraction of total dsDNA on binding matrix applied. Our destruction-extraction procedure is extremely quick and efficient providing dsDNAs of high purity and integrity, free of PCR inhibitors. Advantages to conventional...

PCR usually consists of a series of 20 to 40 cycles to amplify specific regions of a DNA strand in an amount sufficient for detection. Each cycle of amplification includes three steps, each double stranded (dsDNA) melts into single strands (ssDNA) their complementary complementary copies primers with dNTPs Sample is cooled slowly Primers bind after three cycle ADVANTAGES OF PCR METHOD IN DETECTING MICROBIAL CONTAMINATION AS COMPARED TO CONVENTIONAL MICROBIOLOGY METHODS: • Short assay time (2-3 hours) • Direct detection of infectious agents • Elimination of false negative results from...

DIAGON real-time PCR kits Qualitative and quantitative analysis of microbial contaminants in diverse samples DIAGON real-time PCR enables to monitor the kinetics of amplicon accumulation during cycling time. Fluorescence liberated from the reporter (R) dye when separated from the quencher (Q) dye is detected as an indirect measure of the initial amount of Shelf life: 6 months Fluorecence quenching Fluorescent-labelled Probe Liberated fluorescence Primer extension • Amplification of target gene together with regulatory elements to increase selection of functional active microbes • Detection...

DIAGON rt PCR kits...new technologies for increased specificity and platform independance Spectral Shift for real platform PCR fluorescence maxima reading occurs within well defined limits of emission wavelength maxima, usually within 5-10 nm. This means that other platforms cannot or only weakly read fluorescence maxima from the same probe. Diagon kits contain a special red shift component that makes the probe's emission maximum plateau-like with 15-25 nm bandwidth. This make Diagon PCR kits realy platform independent and suitable for wide appli- Diagon CAMEL probe concept for maximum...

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e-mail: diagon@diagon.com PT. Inti Diagontama Selaras - Indonesia Perkantoran Plaza Pasifik A3/59 - Jl. Raya Boulevard Barat Kelapa Gading Permal Jakarta 14250 e-mail: dsu@centrin.net.id DIAGON Ltda. - Brasil Av. Jose Candido Da Silveira, 2100 Belo Horizonte, e-mail: diagon@diagon.com.br