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SYNTAX 96 STANDARD KITS

SYNTAX 96 STANDARD KITS

SYNTAX 96 STANDARD KITS

Product catalog summary
Overview: The document provides detailed information about the SYNTAX DNA Synthesis Kit and related products, designed for synthesizing DNA oligos. It outlines specifications, procedures, and components necessary for effective use.
Specifications: The SYNTAX DNA Synthesis Kit allows for the synthesis of up to 96 unlabeled or labeled DNA oligos ≤80nt in one run, or up to 384 oligos ≤30nt in four runs. The synthesis process is rapid, with setup taking 15-30 minutes and completion in 6-17 hours depending on oligo length. The kit supports the inclusion of degenerate bases and offers a variety of labeling options including fluorophores, quenchers, and biotin.
Procedures: The synthesis process requires the SYNTAX 96 Standard Run Kit, which includes necessary reagents and consumables. The oligos produced are desalted, normalized, and ready for immediate use in applications such as PCR, qPCR, and sequencing.
Kit Components: The kit is divided into three boxes containing synthesis reagents, nucleotides, enzymes, and post-processing reagents. Storage conditions vary, with some components requiring refrigeration or freezing.
Label Specifications: The document details the peak excitation and emission wavelengths for various fluorophores and their compatible quenchers. These labels are sourced from ATTO-TEC GmbH.
Additional Information: The document includes contact information for DNA Script's US and European headquarters and notes that the products are for research use only.
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Catalog excerpts

SYNTAX 96 STANDARD KITS-1

SYNTAX 96 STANDARD KITS FOR USE WITH THE SYNTAXTM SYSTEM, MODEL STX-200 Cat. No. 101867 SYNTAX DNA Synthesis Kit Cat. No. 101869 SYNTAX 96 Standard Run Kit Cat. No. 101871 SYNTAX 96 Standard Run Kit, Custom iDNA, pack of 8 The SYNTAX 96 Standard Kits include the reagents and consumables required for the parallel enzymatic synthesis of 96 ready-to-use DNA oligos of up to 80nt in length and are intended for use in applications such as PCR, qPCR, Sanger sequencing, protein engineering, or target enrichment. Rapid setup and printing of oligos enable same or next-day use in downstream workflows. The synthesis and run kits are both required for oligo synthesis using the SYNTAX System. HIGHLIGHTS Flexible runs: Synthesize up to 96 unlabeled or labeled DNA oligos ≤80nt in one run or up to 384 oligos ≤30nt in four* runs using the SYNTAX DNA Synthesis Kit. Each run requires one SYNTAX 96 Standard Run Kit. SYNTAX 96 STANDARD KIT SPECIFICATIONS Turnaround time • Approximately 6 hours for 96 x 20mer oligos • Approximately 17 hours for 96 x 80mer oligos Oligo length Bases Labels Degenerate Bases Yield and concentration (labeled oligos) Error rate (per base) Unmodified A, T, G, and C Fluorophores – Green, Yellow, Orange, Red, Deep Red Quenchers – Quencher 1, Quencher 2, Quencher 3 Biotin ** For oligos determined to be of standard synthesis difficulty by the Console Software. Label options: Menu of 5 fluorophores, 3 quenchers, and biotin. Degenerate Bases: Ability to include degenerate bases in the sequence to synthesize degenerate oligos. SYNTAX LABEL SPECIFICATIONS*** Fluorophore Peak Excitation Peak Emission Fast setup and synthesis: Set up a run in approximately 15-30 minutes and oligos are ready in 6-17 hours depending on length. Use with TAMRA channel Application-ready output: Oligos are desalted and normalized in molecular biology-grade water and ready for immediate use in downstream applications. * Runs must be performed within 7 days. *** The SYNTAX Labels contain fluorophores from ATTO-TEC GmbH: Yellow (532) contains ATTO 532, Orange (550) contains ATTO 550, Red (565) contains ATTO 56

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SYNTAX 96 STANDARD KITS-2

KIT COMPONENTS SYNTAX DNA Synthesis Kit Synthesis Reagents Box 1 of 3 • Includes the S1 and S2 synthesis reagents for the SYNTAX DNA Synthesis Kit • Store at 4°C (shipped at room temperature) • Includes the S3 and S4 synthesis reagents for the SYNTAX DNA Synthesis Kit • Store at room temperature (shipped at room temperature) Synthesis Nucleotides & Enzyme Box 3 of 3 • Includes the nucleotides and enzyme reagents for the SYNTAX DNA Synthesis Kit • Store at -20°C (shipped on dry ice) SYNTAX 96 Standard Run Kit (and Custom iDNA version) Post-Processing Reagents Box 1 of 3 • Includes the post-processing...

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