Product information AllPhase human MSC culture kit REF Supplement: -20BC Protect from light Phenol Red Yes This product was manufactured for R&D use only. It is not intended for injection or diagnostic procedures. Do not use AllPhase human MSC culture kit beyond the expiration date indicated on the products. AllPhase human MSC Medium is an optimized serumfree and xeno-free medium recommended for the growth and expansion of ex vivo human mesenchymal stem cells (hMSCs), including bone marrow mesenchymal stem cell (BM-MSC), adipose-derived stem cell (ADSC) and umbilical cord-derived mesenchymal stem cell (UC-MSC). Human MSCs cultured with AllPhase human MSC Medium keep an excellent expansion rate after several passages and maintain their self-renewal and multi-lineage differentiation potential in coating-free condition. AllPhase Supplement is provided in liquid form (10 ml) and should be used to prepare complete AllPhase MSC medium (500 ml). Follow the procedure as specified in the user manual to prepare AllPhase complete medium. AllPhase Supplement should be stored at -20ЂC in the dark. To ensure stability, AllPhase Supplement should be thawed at 4ЂC for 20-24 hours before use. Do not refreeze. AllPhase human MSC Medium should be stored at 2ЂC to 8ЂC. Тݖ Aseptically add 10 ml of AllPhase Supplement in 500 ml of basal medium. AllPhase Supplement performance is tested for the maintenance and expansion of adipose-derived stem cells for 3 passages. Doubling time ≤ 35 hours. Others tested parameters: pH (7.0-8.0), endotoxins (<1 EU/ml), mycoplasma and sterility. No.18, Ln.10, Taiping 21st St., Taiping Dist., Taichung City 41161, Taiwan (R.O.C.) (+886) 04-22766193 www.duo