GroPep Bioreagents Human IGF-II Polyclonal Antibody (Rabbit) Description Human IGF-II polyclonal antibody was prepared by immunising rabbits with a human IGF-II conjugated to ovalbumin. The immunoglobulin fraction was purified by Protein A affinity chromatography. The antibody cross-reacts 100% with human and porcine IGF-II; 96% with chicken IGF-II; 91% with rat IGF-II and 85% with bovine IGF-II. Cross-reactivity to human, chicken and rat IGF-I is < 0.1%. It does not cross react with insulin from a range of species. The antibody is suitable for use in immunoassay, immunoblotting and immunohistochemistry. References: Immunogen: Source: Appearance: Storage/Stability: Owens PC et al (1990) Journal of Endocrinology 124, 269-275 Human IGF-II conjugated to ovalbumin Rabbit antiserum. The immunoglobulin fraction was purified by Protein A affinity chromatography of serum obtained from a rabbit immunised with a recombinant human IGF-II conjugated to ovalbumin. Human and porcine IGF-II 100%. Chicken IGF-II 96%, rat IGF-II 91%, bovine IGF-II 85%. Human, chicken and rat IGF-I < 0.1%. No cross-reactivity to insulin Lyophilized powder At least 2 years at 2 - 4°C (lyophilized). After reconstitution store at -20°C or -80°C Avoid freeze-thaw cycles Dissolve the vial contents in 400 pl sterile water or buffer solution and stand for 10 minutes at room temperature. Dilute further in a buffer suitable for the application. Suitable for use in immunoassay, immunoblotting and immunochemistry. Each application and titre should be determined in house. As a guideline, following reconstitution a dilution in the range 1:500-1:32,000 should give a suitable absorbance in a conventional immunoassay format. Product Codes and Pricing Polyclonal Antibody Related Products: IGF-II Polyclonal Antibody (Rabbit) Human IGF-II (Receptor grade) Chicken IGF-II Rat IGF-II GroPep Bioreagents Pty Ltd 51 West Thebarton Road Thebarton SA 5031 Australia Postal Address: PO Box 10065 Adelaide Business Centre SA 5000 Australia Email: [email protected] Internet: www.gropep.com Eagle Biosciences. Inc. 20A NW Bi.vd. Suite 112 Nashua. NH 03063 P 617-419-2019 p 617-419-1110 WWW.EAGLEBlO.COM — [email protected]