ReCap™ Capillary Array Regeneration kit for ABI3130 series Genetic Analyzer Quick Reference Guide Version: 1.0 Revision date: 06 SEP 2018 Product and Company Information Product name: ReCapTM Capillary Array Regeneration kit for ABI 3130 series Genetic Product use: For Research Use Only Company: Telephone: Email: NimaGen BV Lagelandseweg 56 6545 CG Nijmegen The Netherlands +31 (0)24 820 02 41 [email protected] ReCapTM Capillary Array Regeneration kit (v1.0)

Introduction The ReCapTM Capillary Array Regeneration kit offers a complete rejuvenation and revitaLization of oLd and faiLing capiLLaries. The kit can be used on capillary arrays that start to Lose resolution, or showing smears or taiLs in the eLectropherogram. AdditionaLLy, it cLeans the pump bLocks of the machine, preventing or getting rid of yeLLow or red haze. Warning: Use good laboratory practice and wear proper protectives, including gloves, goggles and lab coat when handling the buffers supplied in this kit. Wash body parts with ample amount of water immediately if they come in contact...

1. Start the computer and instrument and Launch data coLLection software 2. Open the doors and remove the Left buffer jar from the pump bLock, Make sure the white drip tray is in pLace underneath the pump bLock 3. FiLL the empty buffer bottLe compLeteLy with deionized water and instaLL it at the poLymer bottLe position 4. Perform and compLete a water wash wizard from the wizard puLL-down menu ✓ When toLd to reinstaLL poLymer, do not repLace the bottLe. Choose for “same Lot". NOTE: When the system during the wizard asks for bubbles visible, always click “no", even if there are bubbles. Because...

NOTE: Similar to a new capillary array, it may take up to two runs for the array to reach optimal, performance. Published by: NimaGen B.V. Lagelandseweg 56 NL-6545CG Nijmegen The Netherlands www.nimagen.com © 2018 NimaGen All rights reserved. ReCapTM Capillary Array Regeneration kit (vi.0)