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The RT-qPCR for the detection of SARS-CoV-2 RNA

The RT-qPCR for the detection of SARS-CoV-2 RNA

The RT-qPCR for the detection of SARS-CoV-2 RNA

Product catalog summary
Overview: The document provides detailed information about the EliGene COVID-19 detection kits supplied by ELISABETH PHARMACON. These kits are designed for the detection and confirmation of SARS-CoV-2.
Specifications: The kits include the EliGene SARS-CoV-2 BASIC A RT and BASIC A500 RT for primary detection, and the EliGene SARS-CoV-2 CONFIRM RT for confirmation and specification of unclear samples. The BASIC kits do not require confirmation due to their 100% specificity.
Procedures: Each kit is a quadruplex RT-qPCR system testing four targets simultaneously in one tube. This ensures positive results even in case of virus mutation and contributes to high sensitivity, detecting less than 15 RNA copies. The fourth target is an internal RNA-based amplification control.
Standards and Compatibility: All kits are CE IVD certified and applicable to various clinical and environmental samples. They use TaqMan probe technology and are compatible with devices like LightCycler 480, RotorGene-Q, CFX96, and QuantStudio. They are also compatible with the EliGene Viral RNA/DNA FAST Isolation Kit for rapid RNA/DNA isolation.
Recommendations: The document recommends using the BASIC kits for primary detection and the CONFIRM kit for confirmation in cases of unclear results or contamination. The kits are designed to ensure user comfort and clear interpretation of results.
Key Features: The BASIC A RT kit is for 100 samples, while the BASIC A500 RT kit is for 500 samples, offering space and cost efficiency. All kits are verified monthly against the GenBank database to ensure functionality.
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Catalog excerpts

The RT-qPCR for the detection of SARS-CoV-2 RNA-1

SARS-CoV-2 detection kits supplied by ELISABETH PHARMACON We recommend the EliGene® SARS-CoV-2 BASIC A RT and EliGene® SARS-CoV-2 BASIC A500 RT kits for primary detection. We advise using the EliGene® SARS-CoV-2 CONFIRM RT for con irmation, speci ication of unclear samples or in the case of laboratory contamination. Positive results obtained with EliGene® SARS-CoV-2 BASIC A RT and EliGene® SARS-CoV-2 BASIC A500 RT need not be con irmed as the speci icity of both kits is 100%. www.elisabeth.cz E: [email protected] | T: +420 542 213 851 ELISABETH PHARMACON, spol. s r.o. Rokycanova 4437/5, Brno-Zidenice

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The RT-qPCR for the detection of SARS-CoV-2 RNA-2

Common to all kits ź Each kit is a quadruplex RT-qPCR system, therefore four targets are simultaneously tested in one tube. The three targets amplify different SARS-CoV-2 speci ic sequences, however they all are detected in a single channel. ź This procedure ensures positive results even in case of virus mutation and was chosen with regard to the clear interpretation of results and user comfort. At the same time, such a format contributes to the high sensitivity of a method that was determined by testing (not empirically) to less than 15 copies of RNA added to the sample. ź The most sensitive...

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