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FUNGIFAST® AFG Brochure

FUNGIFAST® AFG Brochure

Product catalog summary

Overview: The document describes the FUNGIFAST® AFG, a colorimetric broth microdilution method for antifungal susceptibility testing of yeasts. It is designed for routine use and provides results within 24 hours.

Key Features:

Uses a modified RPMI medium.
Tests main systemic antifungals: Amphotericin B, Flucytosine, Itraconazole, Fluconazole, and Voriconazole.
Easy-to-read results with a visual colorimetric method.
Disposable tray with dehydrated antifungal agents and serial two-fold dilutions.
Results interpreted as MIC or breakpoints.

Methodology:

Preparation of the Inoculum: Isolated colonies are transferred to the SUSPENSION FUNGI vial and standardized to 2 McFarland.
Negative Control: 100 µl of MES FUNGI is transferred into the negative control well.
MES Inoculation: 10 µl of the suspension is transferred into the remaining MES FUNGI.
Tray Inoculation: Inoculated MES FUNGI and paraffin oil are added to the tray wells.
Incubation: Incubate at 37°C for 24-72 hours.
Results: Growth is indicated by color change, with clear endpoints for interpretation.

Benefits:

Rapid results within 24 hours for most yeasts.
Convenient with individual packaging and ready-to-use reagents.
Cost-effective with long reagent storage and no need for instruments.

Product Information:

SUSPENSION FUNGI: Buffered agar medium for inoculum preparation.
MES FUNGI: Modified RPMI medium for testing.
TC FUNGI: Barium sulphate solution for turbidity control.
FUNGIFAST AFG tray: 20-well tray for testing one sample.

Quality Assurance: The method shows agreement with the NCCLS standard method, evaluated in a specialized mycology laboratory.

Contact Information: ELITech France SAS, with contact details provided for further inquiries.

Catalog excerpts

Antifungal Susceptibility Testing of Yeasts A colorimetric broth microdilution method for routine use RPMI modified medium Main systemic antifungals Easy to read Results within 24 hours INTERNATIONAL MICROBIO

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FUNGIFAST® AFG Routine use method Disposable tray Dehydrated antifungal agents Serial two-fold dilutions MIC or breakpoints interpretation Main antifungal agents tested (Amphotericin B, Flucytosine, Itraconazole, Fluconazole, Voriconazole) Quality method Agreement between FUNGIFAST AFG and the NCCLS standard method evaluated in a specialized mycology laboratory Easy to read, easy to interpret Redox indicator Positive and negative controls Clear-cut endpoint results Ready to use result sheets Products SUSPENSION FUNGI: Buffered agar medium for the dilution and standardization...

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