NUCLEIC ACID EXTRACTION Magnetic cfDNA Extraction Kit Bottle format 96B format CE, FDA, NMPA Registered 1. Description EmerTher® Magnetic Circulating Free DNA Extraction Kit is used for extracting circulating free DNA (cfDNA) from biological samples (e.g. plasma) with high efficiency and is an ideal sample preparation kit for Noninvasive Prenatal Testing (NIPT) and liquid biopsy. The experiment procedure is simple and efficient: 1) add sample to a lysis-binding solution to enable the binding of cfDNA to magnetic beads; 2) apply magnetic force enabling easy wash of the beads with buffers; 3) elute cfDNA from the beads using an elution solution. Purified cfDNA can be directly used for gene sequencing (such as NIPT or liquid biopsy), library construction, and other downstream applications. 2. Features High throughput: Fast extraction, complete in < 1 hr Flexible sample volume: excel at extraction of a trace amount of cfDNA from samples (200 µL to several mL). Sample extraction process is scalable for large volumes. CfDNA peak resides at ~170-200 bp with high purity and high concentration Automation: compatible with a variety of automated magnetic bead processors; pre-filled plates are provided if needed. The EmerTher Company Phone： 1-800-868-0176 (USA) EmerTher superparamagnetic beads have a solid core consisting of iron oxide clusters, coated with a hydrophilic layer, making it a superior tool to purify biomolecules over similar products. Uniform nano-superparamagnetic bead mass production capacity + optimized technology and reagent formulation: Email: [email protected] Reliable and Efficient Extraction, Originated from Core Technologies Website: www.emerther.com US Site Address: 622 NJ-10, Suite 18B, Whippany, NJ 07981, US

NUCLEIC ACID EXTRACTION cfDNA extracted from 23 plasma samples (5 cancer patients and 18 healthy volunteers, 1 mL/sample) with EmerTher cfDNA extraction kit and quantified by AccuBlue DNA quantitation Distribution of extracted cfDNA analyzed by Agilent 2100 bioanalyzer and Qseq capillary electrophoresis cf DNA content in normal population（~10 ng/mL) and cancer patient (~100 ng/mL） Evaluation of the recovery of small DNA fragments 0.01 ng and 1 ng small DNA fragments were added to bovine serum (3 mL), which were then extracted using EmerTher cfDNA extraction kit; the fluorescence quantitative...

NUCLEIC ACID EXTRACTION 4. Components of the Kits Catalog No. Total sample volume 12 mL (20 preps, calculated based on 600 µL sample/prep) Total sample volume 60 mL (100 preps, calculated based on 600 µL sample/prep) Protein digestion solution Magnetic bead suspension Nucleic acid binding buffer Washing solution II (75% ethanol in water), prepared by user. Elution solution (TE buffer pH7.5-8.5 or ddH2O), prepared by user. 5. Compatible instruments Prefilled plates contain all necessary extraction reagents, plates and tip combs; ready to be used on most automatic nucleic acid extraction instruments/liquid...

NUCLEIC ACID EXTRACTION Founded in 2010, the EmerTher Company is an IVD manufacturer specialized in the development and manufacture of nano-scale superparamagnetic beads and related products for biomedical applications. We provide a number of high-quality, cost-effective products and ready-to-use solutions for biological sample collection, nucleic acid extraction, and protein purification, including： • 3 viral transport media (non-inactivated; inactivated; inactivated without guanidine) and 3 swabs • 20+ magnetic bead-based nucleic acid extraction kits • 6 protein purification magnetic beads...