• 10 TotalTarget Strips packed in a moisture-resistant canister • 10 reaction tubes (clear) • 10 dilution tubes (blue) • Multi-Matrix Barcode Card -kit lot specific •Venvirologix Total Target™ Kit* for Gluten Catalog AT-Gluten-C Part #13028 The TotalTarget Kit for Gluten Detection is designed to quickly extract and quantitate gluten in corn, corn meal, corn grits, and corn flour; masa flour; rice; sorghum; and soy.The TotalTarget Kit will detect gluten protein with the limit of quantitation at 4 ppm, when used in conjunction with the QuickScan System. The range of this kit detects gluten up to 35 ppm. • It is strongly recommended that you use a dedicated grinder for your Gluten testing that isn't used to grind gluten containing grains (Wheat, Rye, Barley, Triticale) to avoid potential contamination. If you are using a blender for grinding, make sure to use thoroughly cleaned cups, blades, gaskets, and collars. • Before testing, the enclosed Multi-Matrix Barcode Card (MMBC) must be scanned just once for each kit lot to upload information to the QuickScan. The software will prompt users to select a Matrix Group (MG) before proceeding to the result screen. • QuickScan Software Version 5.6 or later is required. • Store test strips at 4°C (39.2°F) as much as possible. Only remove test strips needed and securely reseal the storage canister before putting back into refrigeration. A composite sample is collected, ground, and extracted to solubilize any gluten residues present. The extract is diluted into a buffer before being run on the test strip. Each strip has an absorbent pad at each end. The sample extract travels up the test strip and is absorbed into the larger pad at the top of the strip. At the end of the reaction time, the strip is cut at the top of the arrow tape, the bottom pads are discarded, and the strip is inserted into the QuickScan reader to obtain traceable, quantitative results. Cut here - • QuickScan System* • Bunn grinder or equivalent • 20-mesh screen (available through Seedburo or other vendor) • Digital scale for weighing samples • Extraction cups with lids* or other suitable vessels for sample extraction • Graduated cylinder* • Orbital/rotary shaker • 1 mLadjustable pipette to deliver 500 pL/1000 pL/tips* • Distilled, deionized or bottled water *Available Accessories: Item Catalog No. Part # Case of500; for extracting samples up to 30g Graduated cylinder (100 mL) MiniPet pipette 100 pL (one/location free) Microcentrifuge 1 mL adjustable pipette Incubator Coffee filters (100) Pipette tips for 1 mL pipette (50) Coffee filters*

TotalTarget Gluten Detailed instructions for use of the QuickScan System are supplied with each unit and can also be found at www.envirologix.com/support. The lot-specific Multi-Matrix Barcode Card (MMBC) must be scanned into the system prior to testing. Strips inserted into the reader are read by touching or clicking on the “Read Test” button on the screen. The “Select Matrix Groups” screen will appear if more than one barcode was scanned into the system from the MMBC. Select the group that displays the matrix run. Results are then recorded in an electronic worksheet, allowing each user to report...

TotalTarget Gluten Sample Extraction A Whole corn / co-products, rice and sorghum (25g sample) - The whole corn extraction process is the same as used for AQ-309-BG TotalTox Aflatoxin A1. Add 1 packet EB17 to the sample, measure the water (distilled, deionized or flat (non-carbonated) bottled water) with a graduated cylinder or other appropriate measuring device, and add 75 mL to the sub-sample in the extraction vessel. A2. Cap sample cup tightly, shake by hand vigorously for 10 seconds to ensure proper wetting of the ground sample, and place on shaker at the highest speed (> 300 rpm) for 1 minute,...

TotalTarget Gluten Note: Scanning the Multi-Matrix Barcode Card once per kit lot is required. The QuickScan software will prompt users to select a Matrix Group (MG) before proceeding to the result screen. 1. Place the strip into the reaction vial containing the final sample extract. The arrow tape on the end of the strip should point into the reaction vial. 2. Sample extract will travel up the strip (flow may not be visible immediately—this is expected and normal). 3. Allow the strip to develop for 5 minutes. Immediately cut off and discard the bottom section of the strip covered by the arrow...

TotalTarget Gluten Page 5 of 9 LIMITED WARRANTY EnviroLogix Inc. (“EnviroLogix”) warrants the products sold hereunder (“the Products”) against defects in materials and workmanship when used in accordance with the applicable instructions for a period not to extend beyond a product’s printed expiration date. If the Products do not conform to this Limited Warranty and the customer notifies EnviroLogix in writing of such defects during the warranty period, including an offer by the customer to return the Products to EnviroLogix for evaluation, EnviroLogix will repair or replace, at its option, any...

TotalTarget Gluten Safety data sheet SECTION 1. Identification of the substance/mixture and of the company/undertaking J.l Product identifier Part number. 1.2 Relevant identified uses of the substance or mixture and uses advised against. 1.3 Details of the supplier of the safety data Manufacturer Supplier 1.4 Emergency telephone number: SECTION 2. Hazards Identification. 2.1 Classification of the substance or mixture Classification according lo OSHA 29CFR 1910.1200 and Regulation EC 1272/2008 (CLP): Labeling according to OSHA 29CFR 1910 1200 and Regulati (EC) 1272/2008 Hazard pictograms Signal...

TotalTarget GlutenPage 7 of 9 Rev. 3 •"•'ENVIROLOGIX SECTION 15. Regulatory information. 15.1 Safety, health and environmental regulations Health hazard: 2 . Flammability: 1 , Physical Hazards. 0 Health and Safety Reporting List CERCLA SARA Section 502 (Extremely Hazardous TSCA 8(b) inventory. Sodium laurvl sulfate Clean Air Act Clean Water Act OSHA Europpan/lnternatiaual Regulations European labeling in accordance with EC Directives 15.2 Chemical safety assessment SECTION 16. Other Information. This product is on the European inventory of Existing Commercial Chemical CLASS D-2B Material causing...