The 3D-Bioplotter® Process Unique to the 3D-Bioplotter® 4th Generation 3D-Bioplotter® Manufacturer Series 4th Generation 3D-Bioplotter® Developer Series Key Features of the 3D-Bioplotter® Application: Bone Regeneration Application: Drug Release Application: Cell/Organ Printing & Soft Tissue Fabrication Other Applications

3D Bioprinting - The Future Is Now! The EnvisionTEC 3D-Bioplotter® system has been used since 2000 for a variety of medical applications. Most research done to date using our machines has been in the pre-clinical setting, yielding many publications by pre-eminent scientists from the materials science, neuroimaging, and toxicology disciplines. In the clinical setting, patient CT or MRI scans are used to create STL files to print solid 3D models which can then be used as templates for implants. Tissue Engineering and Controlled Drug Release require 3D scaffolds with well-defined external and internal...

3D non-woven with interconnecting pores A liquid, melt, paste or gel is dispensed from a material cartridge through a needle tip from a 3-axis system to create a 3D object. The material to be used must, through a physical or chemical reaction, solidify. The widest range of materials of any 3D printing technology can be processed.

Unique to the 3D-BIOPLOTTER® Process Uses raw materials (powder, pellets, etc.) without requiring a preprocessed filament. Medical-grade materials can be used. Designed for use in a sterile biosafety cabinet with built-in sterile and particle filters for the input compressed air. Materials are kept in sterilizable cartridges, thus avoiding touching the machine: easier to clean and sterilize. Each customer can create their own processing parameters. Not locked to any proprietary materials, customers can choose their prefered vendors, as well as required medical grades, mixture compositions and...

Manufacturer Series • Designed both as a tool for advanced Tissue Engineering research, as well as for use in a production environment. • Capable of using all hardware and software options of the 3D-Bioplotter Series. • Includes heated platform and sterile filter, recommended for Cell Printing / Organ Printing.

Machine Specification Manufacturer Series EnvisionTEC - German Precision

4th Generation 3D-Bioplotter® Developer Series • Designed for research groups new to the field of Tissue Engineering, as well as for specialized use, where the limited capability may still meet requirements. • Consisting of the same basic hardware and software as the Manufacturer Series, but with reduced functionality regarding camera, build platform and park positions. • Not upgradable to the same capability of the Manufacturer Series.

Machine Specification Developer Series EnvisionTEC - German Precision

Key Features 3D-Bioplotter® Input of outer shapes through STL files. Multi-part and multi-material capable through the use of an automatic tool changer and multiple print heads. Database of inner patterns (user-editable) in the controlling software, avoiding requiring patterns in the STL files. Database of materials (user-editable) with all process parameters. Individual temperature control of each printing head, both in the parking positions, as well as during printing. 2D Dot-Printing (Biopatterning) capability. Complete control of all printing parameters (temperature, pressure, speed, etc)...

Low Temperature Print Head (0°C to 70°C) with disposable PE cartridges. High Temperature Print Head (30°C to 250°C) with reusable stainless steel cartridges. Automatic Platform Height Control for Petri Dishes, Well Plates, as well as other printing surfaces. UV Curing Head (365 nm). Needle cleaning station, with automatic cleaning before and during the print project available. Luer Lock needle tips, 0.1mm to 1.0mm inner diameter available. LOG file creation after project completion with all relevant data. Footprint (L x W x H): 976 x 623 x 773 mm (38.4” x 24.5” x 30.4”) Weight: 130 kg (286.6...

Application: Bone Regeneration Ceramic/Metal Pastes Tricalcium Phosphate Phase Transition Sample Papers: • Li, J. P., et al. „The effect of scaffold architecture on properties of direct 3D fiber deposition of porous Ti6Al4V for orthopedic implants.“ Journal of Biomedical Materials Research Part A 92.1 (2010): 33-42. Haberstroh, Kathrin, et al. „Bone repair by cell-seeded 3D‐bioplotted composite scaffolds made of collagen treated tricalciumphosphate or tricalciumphosphate-chitosan-collagen hydrogel or PLGA in ovine critical‐sized calvarial defects.“ Journal of Biomedical Materials Research Part...

Application: Drug Release Thermoplasts PCL PLLA PLGA Phase Transition Sample Papers: • Kammerer, M., et al. „Valproate release from polycaprolactone implants prepared by 3D- bioplotting.“ Die Pharmazie-An International Journal of Pharmaceutical Sciences 66.7 (2011): 511-516. Yilgor, P., et al. „An in vivo study on the effect of scaffold geometry and growth factor release on the healing of bone defects.“ Journal of tissue engineering and regenerative medicine 7.9 (2013): 687-696. Yuan, Jing, et al. „The preliminary performance study of the 3D printing of a tricalcium phosphate scaffold for the...

Application: Soft Tissue Fabrication Cell Printing & Organ Printing Hydrogels Agar Hyaluronic Acid Phase Transition Sample Papers: • Maher, Paul S., et al. „Thermal imaging analysis of 3D biological agarose matrices.“ International Journal of Medical Engineering and Informatics 3.2 (2011): 167-179. Billiet, Thomas, et al. „The 3D printing of gelatin methacrylamide cell-laden tissue-engineered constructs with high cell viability.“ Biomaterials 35.1 (2014): 49-62. Chien, Karen B., et al. „In vivo acute and humoral response to three-dimensional porous soy protein scaffolds.“ Acta Biomaterialia 9.11...

Other Applications Other Materials Polyurethane Phase Transition Sample Papers: • Kiziltay, Aysel, et al. „Poly (ester‐urethane) scaffolds: effect of structure on properties and osteogenic activity of stem cells.“ Journal of tissue engineering and regenerative medicine (2013). Bakarich, Shannon E. “Three-Dimensional Printing Fiber Reinforced Hydrogel Composites.” ACS Appl. Mater. Interfaces 6.18 (2014): 15998-16006. Nathan‐Walleser, Teressa, et al. „3D Micro‐Extrusion of Graphene‐based Active Electrodes: Towards High‐Rate AC Line Filtering Performance Electrochemical Capacitors.“ Advanced Functional...