Swift SpectrumTM 48 Real Time Thermal Cycler Spectrum 48 Real Time Cycler Swift Spectrum Series Combines Amplification and Detection Polymerase Chain Reaction (PCR) is an important tool for the detection of DNA and RNA with applications across all Biotechnology / Molecular Biology research, including forensics, gene expression, identification and discovery. Traditional PCR has relied on Agorose gel-based end point detection of the reaction, in the plateau phase. This has been time consuming, not very precise and with a degree of sample to samples variability. Real time PCR combines the amplification and detection steps into one single procedure, saving time. Real time PCR measures sample during the Exponential phase of the PCR reaction and produces results while the reaction is in progress. This achieves detection accuracy from very low initial concentrations of starting material. Real Time PCR is sufficiently sensitive to detect as low as 2-fold changes, allowing discrimination in yields that are not possible to achieve with Agarose gels. • In Real Time PCR, a fluorescent label is added to the PCR reaction. • By measuring the increase in fluorescence during the exponential phase of PCR, the starting amount of nucleic acid can be quantified. The Swift Spectrum Real Time Thermal Cycler monitors the reaction mixture and measures fluorescence during each round of amplification, not just at the end point. • There is a quantitative relationship between the amount of starting material and the amount of PCR product at any specific cycler number. RT-PCR detects the accumulation of amplicon during the reaction and when the flourescence intensity of the reaction exceeds the background fluorescence (Ct Value) the amplification can be visualized. • When samples of known starting amounts are simultaneously amplified under the same conditions as the unknowns, it is possible to construct a standard curve. From this curve, the investigator can determine amplicon concentrations. Final steps in a PCR protocol include visualization and quantification of the amplified nucleic acids. Without real time thermal cycling, this is time consuming, requires additional instrumentation, and limits capacity for detection. Additionally, very low nucleic acid concentrations are sometimes difficult to visualize. Advantages of Esco Swift Spectrum 48 Real Time Thermal Cycler Unlike conventional 96 well block designs, Esco Swift Spectrum 48 Thermal Cycler uses a unique, proprietary block, designed to increase performance and maximize efficiency. • Up to 48 sample capacity with broad range linear detection. • Advanced Peltier and fiberoptic technology achieves accuracy and stability throughout the process. • Uniform heating and cooling throughout the sandwich design, patented sample block delivers highly controlled ramp up, dwell, cool-down and repeat functions critical to sensitive PCR reactions. • This layout guarantees efficient and rapid heat exchange, and, together with the heated lid, eliminates sample evaporation. • The block design minimises the risk of over- and undershoots and guarantees accuracy and temperature uniformity. • This, in turn, increases PCR efficiency and higher template yields, allowing the detection of low concentrations of starting materials. • It is possible to divide the block into 4 segments, allowing the analysis of up to 4 different sample groups.

Features the direct application of established protocols. • Gradient function creates a temperature gradient of 24°C across the sample block. • Volume sensor software automatically adjusts ramp rates to accommodate differences in sample volumes. • A blue light diode for fluorescent dye excitation. • A direct current constant power supply improves thermal efficiency, performance and stability while reducing power consumption by 30% over comparatively sized models. • An automatic hot lid with adjustable temperature. In addition to program and assay set-up, the Swift Spectrum Intuitive software...

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Since Esco was founded in 1978, our company has earned a reputation for innovation in the worldwide laboratory equipment industry. With years of research, R & D and manufacturing experience, Esco Micro Pte Ltd emerged as an innovative global player in the design and manufacturing of peltier-driven thermal cycler products for the life science market. Products sold in more than 100 countries include conventional thermal cyclers, real-time PCR systems and microplate shakers / incubators. Esco Micro Pte. Ltd. • 21 Changi South Street 1 • Singapore 486 777 Tel +65 6542 0833 • Fax +65 6542 6920 • [email protected]...