Swift Spectrum 96 Real Time Thermal Cyclers
4Pages

Swift SpectrumTM 96 Real Time Thermal Cycler Spectrum 96 Real Time Cycler Swift Spectrum Series Combines Amplification and Detection Polymerase Chain Reaction (PCR) is an important tool for the detection of DNA and RNA with applications across all Biotechnology / Molecular Biology research, including forensics, gene expression, identification and discovery. Traditional PCR has relied on Agorose gel-based end point detection of the reaction, in the plateau phase. This has been time consuming, not very precise and with a degree of sample to samples variability. Real time PCR combines the amplification and detection steps into one single procedure, saving time. Real time PCR measures sample during the Exponential phase of the PCR reaction and produces results while the reaction is in progress. This achieves detection accuracy from very low initial concentrations of starting material. Real Time PCR is sufficiently sensitive to detect as low as 2-fold changes, allowing discrimination in yields that are not possible to achieve with Agarose gels. • In Real Time PCR, a fluorescent label is added to the PCR reaction. • By measuring the increase in fluorescence during the exponential phase of PCR, the starting amount of nucleic acid can be quantified. • When samples of known starting amounts are simultaneously amplified under the same conditions as the unknowns, it is possible to construct a standard curve. From this curve, the investigator can determine amplicon concentrations. Final steps in a PCR protocol include visualization and quantification of the amplified nucleic acids. Without real time thermal cycling, this is time consuming, requires additional instrumentation, and limits capacity for detection. Additionally, very low nucleic acid concentrations are sometimes difficult to visualize. Advantages of Esco Swift Spectrum 96 Real Time Thermal Cycler The Real-Time PCR 96 System combines new technology of an unprecedented range of options and features with unsurpassed data quality, all at an affordable price. The Real-time PCR 96 has the unique patented block dissipation technology and bottom detection mode, which provide better heating/cooling rate, temperature accuracy, uniformity and stability. Adopting Ferrotec newest Peltier, fiber optic technology and a new global wide range power supply, the highly improved instrument is available for a variety of scientific research and clinical applications. The Swift Spectrum Real Time Thermal Cycler monitors the reaction mixture and measures fluorescence during each round of amplification, not just at the end point. • Up to 96 sample capacity with broad range linear detection. • There is a quantitative relationship between the amount of starting material and the amount of PCR product at any specific cycler number. RT-PCR detects the accumulation of amplicon during the reaction and when the flourescence intensity of the reaction exceeds the background fluorescence (Ct Value) the amplification can be visualized. • This, in turn, increases PCR efficiency and higher template yields, allowing the detection of low concentrations of starting materials. • The block design minimizes the risk of overshoots & undershoots and guarantees accuracy and temperature uniformity. • It is possible to divide the block into 4 segments, allowing the analysis of up to 4 different sample groups.

• Expanding available. • Becoming less technically demanding. • Multi-competent device for performing with virtually any detection chemistry • PMT detector is one of the most sensitive detectors, and has a fast response time with no need of watercooling. • Affordable pricing when compared with key competitors in the market. • Long Warranty period- 3 years for main body, 2 years for blocks. • Open Platform-Compatible with usage of generic consumables and reagents. Features • The adopted fluorescent real-time detection mode realizes simultaneous amplification and detection in a same tube...

* SPT-RT-96 with 2 PMT and 8 LEDs vs. SPT-RT-48 with 1 PMT and 1 LED Multiple Channels, Open Platform Chemistry Channel Light Cycler Red Quasar 705 * Channel 7 and 8 are preserved dummy channels and may be customized as per customer requirements Technical Specifications, Swift Spectrum 96, Model Number SPT-RT-96 Model Sample Capacity Reaction System Dynamic Range Excitation Wavelength Emission Wavelength FAM, SYBR Green I, Cy3, 5-TAMRA, NED, Cy5, VIC, HEX, TET, JOE, ROD, Texas-Red, Light Cycler Red Quasar 705 Temperature Range Heating / Cooling Rate Temperature Fluctuation ≤+/-0.1°C...

Since Esco was founded in 1978, our company has earned a reputation for innovation in the worldwide laboratory equipment industry. With years of research, R & D and manufacturing experience, Esco Micro Pte. Ltd. emerged as an innovative global player in the design and manufacturing of Peltier-driven thermal cycler products for the life science market. Products sold in more than 100 countries include conventional thermal cyclers, real-time PCR systems and microplate shakers / incubators. Esco Micro Pte. Ltd. • 21 Changi South Street 1 • Singapore 486 777 Tel +65 6542 0833 • Fax +65 6542 6920...