Enzymes & Reagents
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Enzymes & Reagents - 1

Enzymes & Reagents ...for PCR, Real Time PCR and RT-PCR

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Enzymes & Reagents - 2

PCR DNA Polymerases EuroClone recombinant DNA Polymerases are produced and puried from E.coli. Severe and rigorous production procedures ensure the highest quality and the best batch-to-batch consistency. Our Polymerases are always supplied with the specic reaction Buffer. A separate MgCl2 solution is provided with EuroTaq and @Taq. Choose the right enzyme for PCR Use the table below to easy nd the better enzyme for most PCR applications, including special PCR: High Fidelity PCR, “Fast”-PCR or “Direct-Blood” PCR. Amplicon Size Resulting Ends GC–rich templates AT-rich templates High Yield...

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Enzymes & Reagents - 3

PCR EuroTaq Thermostable DNA Polymerase EuroTaq is a traditional thermostable DNA Polymerase for standard PCR reactions. The enzyme is puried from E.Coli PVG-A1 recombinant strain expressing Thermus aquaticus YT1 DNA Polymerase gene. • Ideal for routine PCR • High activity • Maximum exibility for reaction conditions • Higher thermostability *10X Reaction Buffer and MgCl2 Buffer included @Taq Hot Start Thermostable DNA Polymerase @Taq is a recombinant Hot Start Taq DNA Polymerase puried from E.coli PVG-A1. This enzyme is complexed with a monoclonal antibody blocking the polymerase activity...

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Enzymes & Reagents - 4

PCR Master Mix for PCR @Taq Master Mix Hot Start PCR Master Mix This Master mix for Hot Start PCR includes @Taq, the immuno-blocked Taq from EuroClone. It comes in a convenient format of 4 x 50 µl reaction vials, 2X concentrated, for a better handling of the amplication solution. @Taq Master Mix includes all the reagents needed for PCR (Taq Polymerase, reaction Buffer, dNTPs, MgCl2), but not the specic primers. • Hot Start effect • Excellent specicity • High activity level with the benets of a ready-to-use amplication solution • Highly reproducible PCR • Strong reduction of pipetting errors...

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Enzymes & Reagents - 5

Real Time PCR Master Mix for Real Time PCR EuroClone qPCR master mixes are ready-to-use 2X solutions optimized for Real Time PCR. The master mixes include Hot Start @Taq DNA Polymerase and dNTPs in an optimized buffer. The SYBR® Master Mix contains the green intercalating dye allowing DNA detection and analysis without using sequence-specic probes. Only template and primers need to be added. The Master Mix for Probe has been formulated for the detection of amplication products with sequence specic uorogenic probes. Primers, probe and template must be added before use. • Specicity: @Taq Hot...

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Enzymes & Reagents - 6

RT-PCR EuroScript M-MLV Reverse Transcriptase (RNase H -) NOT FOR USA MARKET EuroScript is a genetically modied M-MuLV RT. The enzyme possesses a RNA-dependent and DNA-dependent Polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. It does not degrade RNA in RNA-DNA hybrids during synthesis of the rst strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. EuroScript maintains activity over a wide temperature range (42-55°C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree...

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Enzymes & Reagents - 7

NUCLEOTIDES dNTPs and NTPs EuroClone‘s enzymatic nucleotides manufacturing process and rened purication protocols ensure the highest quality. All our dNTPs and NTPs are ultrapure (> 99%) and quality checked by a set of PCR, RT-PCR and Klenow reactions. EuroClone dNTPs and NTPs have the highest purity, are free of strong PCR inhibiting contaminants as tetraphosphates and pyrophosphates. All lots are checked on HPLC for their purity using a sensitive acetonitrile gradient in 20 mM KH2PO4, 2 mM TBA-SO4 on a Eurospher-100 C18 RP-column (4 x 250 mm). Detection occurs at 254 nm. EuroClone dNTPs...

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Enzymes & Reagents - 8

Diritto d'autore: annete / 123RF Archivio Fotografico Quality Management Systems certied according to ISO 9001 and ISO 13485 international standards Ed0/1114/PR 991 Brochure PCR RT-PCR Ed0/1114/PR 991 Brochure PCR RT-PCR EuroClone S.p.A. Via Figino, 20/22 • 20016 Pero (MI) Italy • +39 02 38195.1 • +39 02 38101465 • info@euroclone.it • www.euroclone.it

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