EUROPE KANEKA EUROGENTEC SA - LIEGE SCIENCE PARK • 4102 Seraing • BELGIUM • Tel.: +32 4 372 74 00 • Fax: +32 4 372 75 00 Toll-free: + 800 666 00 123 • [email protected] • www.eurogentec.com N°RTH AMERICA Kaneka Eurogentec products are sold for research or ANASPEC - 34801 Campus Drive • Fremont, CA 94555 • USA • Tel.: +1-510-791-9560 laboratory use only and are not to be administrated Toll-free: +1 800-452-5530 • Fax: +1 510-791-9572 • [email protected] • www.anaspec.com to humans or used for medical diagnostics. Technical Data Sheet Clone: 27-7 Isotype: IgG1 Host: Mouse Reactivity: IgG1 (heavy + light chains) from camelids Label: None Antibody description Besides the conventional IgGs (IgG1, composed of heavy and light chains), llamas and other camelids produce single chain IgGs (IgG2 and IgG3). They are composed of heavy chains only and are lacking the light chains and the CH1 domains. The antigen binding domain of IgG2 and IgG3, called VhH, is the smallest naturally occurring antibody binding domain. This secondary antibody allows the detection of IgG1 from llamas and other camelids. It was produced in vitro and purified using protein G. It has low to no cross-reactivity toward IgG2 and IgG3. Storage conditions Store at -20°C. Avoid repetitive freeze-thaw cycles. Application The antibody is effective in ELISA, immunoblotting (WB) and other immunodetection methods. Suggested Working Dilution*: ELISA: 2500 to 5000x WB: 2000x (predicted MW: ~150 kDa in PAGE under nonreducing conditions) *The optimal antibody dilution should be determined for each specific assay condition For further information please contact our Customer Help Desk For USA: E-mail: [email protected] Tel.: +1-510-791-9560 • Toll-free: +1-800-452-5530