Monoclonal Antibody ID-MWLRV1-050 • ID-MWLRV1-H50
2Pages

EUROPE LIEGE SCIENCE PARK • 4102 Seraing • BELGIUM • Tel.: +32 4 372 74 00 • Fax: +32 4 372 75 00 • Toll-free: + 800 666 00 123 • info@eurogentec.com • www.eurogentec.com NORTH AMERICA ANASPEC - 34801 Campus Drive • Fremont, CA 94555 • USA • Tel.: +1-510-791-9560 • Toll-free: +1 800-452-5530 • Fax: +1 510-791-9572 • service@anaspec.com • www.anaspec.com Technical Data Sheet iD BlueLadder Revealer - iD BlueLadder Revealer-HRP Monoclonal Antibody ID-MWLRV1-050 • ID-MWLRV1-H50 Eurogentec products are sold for research or laboratory use only and are not to be administered to humans or used for medical diagnostics or for diagnostic purposes. Western Blotting: 1/1000 – 1/5000 suggested dilution. Instruction for Western Blotting application Products Name The iD BlueLadder Revealer is a mouse monoclonal antibody targeting the blue dye of prestained proteins from MW ladders. This antibody can be used to visualize the marker blue bands throughout the whole process of western blotting from gels to membranes and up to X-Ray films. This makes the positioning of the marker on the blot very easy and highly accurate. The antibody is provided alone or coupled to horse radish peroxidase (HRP). 1. Run a SDS-PAGE with a blue pre-stained molecular weight marker and transfer the proteins to a membrane. 2. Block the membrane using an appropriate blocking solution e.g. iD Blocking Kit (ID-WCBLO1-001; Eurogentec). 3. Incubate the membrane with a mix of primary antibodies containing: he primary antibody specific to your protein (for dilution, please refer to a. T the instructions from the manufacturer); b. The iD BlueLadder Revealer (recommended dilution : 1/2000) Note: f the primary antibody specific to your protein is HRP-conjugated, use the I iD BlueLadder Revealer-HRP. This antibody is specific for the blue dye used in prestained protein MW markers. It does not react with human, mouse, rat, chicken, hamster, monkey, yeast and E. coli proteins. 4. the primary antibodies are not HRP-conjugated, incubate the membrane If with secondary antibodies coupled to HRP (or any other conjugate of your choice). An anti-mouse secondary antibody is to be used to detect the iD BlueLadder Revealer (murine primary antibody). 5. Perform the detection according to the conjugate selected. Protein A purified monoclonal antibody. - iD BlueLadder Revealer is delivered in PBS containing 0.05% azide. - BlueLadder Revealer-HRP is delivered in a proprietary buffer for HRP iD stabilization. Note: f HRP is used, a colorimetric or chemiluminescent detection can be i performed using respectively the iD TMB Substrate Kit (ID-SUTMB1) or iD HRP Substrate Kit (ID-SUHRP1) (Eurogentec). Store at -20°C. Avoid multiple freeze-thaw cycles. The iD BlueLadder Revealer and the iD BlueLadder Revealer-HRP are compatible with the iD W

EUROPE LIEGE SCIENCE PARK • 4102 Seraing • BELGIUM • Tel.: +32 4 372 74 00 • Fax: +32 4 372 75 00 • Toll-free: + 800 666 00 123 • info@eurogentec.com • www.eurogentec.com NORTH AMERICA ANASPEC - 34801 Campus Drive • Fremont, CA 94555 • USA • Tel.: +1-510-791-9560 • Toll-free: +1 800-452-5530 • Fax: +1 510-791-9572 • service@anaspec.com • www.anaspec.com Technical Data Sheet Related Products Product Description Molecular Weight Markers iD Multi Color Pre-stained Protein Weight Marker Precasted PAGE Gels iD PAGE Gel, 4-20%, 10 wells Western Blotting Kits iD Western 1H - Enhanced Kit with HRP...