RT-CKFT-18S

Product catalog summary

Storage Conditions
The 18S rRNA Control kit should be stored at -15 °C to -25 °C in a constant temperature freezer, ensuring stability for up to one year.

Kit Contents
The kit includes reagents for up to 250 reactions, featuring a FAM-TAMRA labelled probe for singleplex qPCR and a Yakima Yellow®-Eclipse® DarkQuencher labelled probe for multiplex qPCR.

Procedure
For each 50 µl reaction, add 3 µl of 18S Primer Mix and 1 µl of 18S Probe. The final concentrations are 600 nM for primers and 125 nM for the probe. The kit is designed to detect 18S ribosomal RNA gene across various species, producing a 121 bp amplicon.

Thermocycler Programming
Standard parameters include a 10-minute activation at 95 °C, followed by 40 cycles of 15 seconds at 95 °C and 1 minute at 60 °C. If UNG is used, an additional 2-minute activation at 50 °C is required.

Licensing Information
The product includes limited rights for research use under specific patents. Additional licenses may be required for commercial applications.

Additional Control Kits
Eurogentec offers various control kits for different RNA and DNA targets, including 23S rRNA, 28S rRNA, ß-actin, GAPDH, and others, each with varying expression levels.

Contact Information
For further assistance, contact Eurogentec's customer help desk in Europe or the USA.

Catalog excerpts

Eurogentec Headquarters LIEGE SCIENCE PARK • 4102 SERAING • Belgium • Tel: +32 4 372 76 65 Fax: +32 4 372 75 00 • [email protected] • www.eurogentec.com Eurogentec North America 3347 Industrial Ct • Suite A • San Diego, CA 92121 • USA • Tel: +1 858 793 26 61 Fax: +1 858 793 26 66 • [email protected] • www.eurogentec.com Products and procedures described in this protocol are intended for research purposes only. Storage conditions The 18S rRNA Control kit should be stored at -15 °C to -25 °C in a constant temperature freezer. When stored under these conditions the reagents are stable for 1 year. Procedure We strongly recommend preparation of a reaction mix Each 50 |jl reaction requires the addition of 3 jl of 18S Primer Mix (plain cap) and 1 jl of 18S Probe (brown cap). If required a mix containing the appropriate amount of both primer and probe can be prepared. Dispense 4 jl of this mix per 50 jl reaction. The 18S rRNA Control kit contains enough reagents for up to 250 - 50 jl reactions. Reagent Volume Description 18S primer mix 750 jl (plain cap) 18S probe (brown cap) Human Xsomal DNA 500 jl (red cap) 250 jl One tube of 6.25 jM FAM-TAMRA probe One tube of Human male chromosomal DNA at 30 ng/jl in TE* Final concentration of primers is 600 nM. Final concentration of probe is 125 nM. Prepare an appropriate dilution series of chromosomal DNA in TE or PCR grade water and a no template control. The 18S rRNA Control Kit has been developed to detect the human, rat, mouse or rabbit 18S ribosomal RNA gene (cDNA/ gDNA). The obtained amplicon is 121 bp in length, to guarantee an efficient PCR. Program the Real-Time thermocycler using the following recommended parameters: * 30 ng/jl correspond to copies of 10000 genome copy of 18S (1,5 to 2 milion copies of the gene depending on the individual). The 18S rRNA Control kit FAM-TAMRA be combined with Eurogentec Reverse Transcriptase core kit (first strand reaction kit) reference RT-RTCK-05 followed by Eurogentec qPCR MasterMixes, reference RT-QP2X-03 or qPCR Core kits, reference RT-QP73-05. Alternatively a One step RTqPCR MasterMix Plus, reference RT-QPRT-032X can also be combined with the Control kit. Alternatively a qPCR MasterMix (RT-QP2X-03) can be combined with the control kit for quantitation of gDNA. The kit is available with a FAM-TAMRA labelled probe (RT-CKFT-18S) detected in channel for singleplex qPCR and a Yakima Yellow®-Eclipse® DarkQuencher labelled probe (RT- If UNG is used, program the Real-Time thermocycler using the following recommended parameters: UNG activation 2 min. 50 °C HotGoldStar activation / UNG incativation 10 min. 95 °C 40 Cycles 15 sec. 95 °C CKYD-18S) detected in channel 2 for multiplex qPCR.

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Other Eurogentec Control Kits available: Eurogentec has developped control kits that can be used for either cDNA or cDNA and gDNA. 18S rRNA Control Kit human, rat, mouse, rabbit cDNA and gDNA very high expression level RT-CKYD-18S or RT-CKFT-18S 23S rRNA Control kit E.coli very high expression level RT-CKFT-23S 28S rRNA Control Kit human, mouse cDNA and gDNA very high expression level RT-CKYD-28S U-actin Control Kit human cDNA and gDNA high expression level RT-CKYD-ACTB GAPDH Control kit human cDNA high expression level RT-CKYD-GAPD U-2-microglobulin Control Kit human cDNA and gDNA high expression...

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.

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