FVMPE-RS
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Multiphoton Laser Scanning Microscope FVMPE-RS FLUOVIEW See More Detail at Depth

High Speed Laser Light Stimulation Deep Observation OLYMPUS XL Plan N 25X/1.05WMP °° / 0-0.23 OFN18/WD2 Auto CORR

High-Speed Imaging at Depth The Olympus FVMPE-RS multiphoton imaging system is purpose-built for deep imaging in biological tissue, aimed at revealing both detail and dynamics. Innovative features for efficient delivery and detection of photons in scattering media enable high signal-to-noise ratio acquisition. This translates to bright images with precise details — even from deep within the specimen. High sensitivity is matched with high-speed imaging to capture rapid in vivo responses. For advanced applications, dual-wavelength excitation extending to 1300 nm is available. Independent...

Designed for Deep Imaging Maximize Resolution in Deep Imaging Olympus TruResolution objectives maximize resolution and contrast for 3D imaging deep within thick specimens. The objectives are equipped with a motorized correction collar that can automatically and dynamically compensate for spherical aberration while maintaining focus position. Refractive index mismatch between the sample and immersion medium introduces spherical aberration that degrades the focus of an objective lens. This aberration worsens deeper into the sample, yielding progressively poorer images with depth....

Olympus dedicated multiphoton objectives are optimized for deep imaging under in vivo and cleared tissue conditions. A diverse lineup provides you the opportunity to select an objective according to your research requirements. Different optical designs emphasize high numerical aperture, long working distance, wide field of view, and compatibility with a range of immersion media and tissue clearing agents. Dedicated Multi Photon Objectives The UPLSAPO30XSIR objective applies the efficient IR transmission coating of MPE dedicated objectives to a high numerical aperture silicone oil immersion...

Capture Fast, Dynamic Cellular Processes High-Speed, 438 Frames-per-Second Scanning A fast resonant scanner and conventional galvanometer scanner provide high-speed and high-resolution imaging in a single system. Avoid motion artifacts when imaging dynamic samples with capture rates of 30 fps at 512 × 512 pixels at the full field of view (FN 18) or up to 438 fps at 512 × 32 pixels. These speeds enable applications such as tracking of fast moving cells in blood flow, and observing rapid membrane potential dynamics across neurons and other cells. Galvanometer scanner Resonant scanner High...

A Cooled, High-Sensitivity GaAsP Detector Acquires High S/N Images High signal-to-noise ratio imaging can be acquired even from faint fluorescence through the use of high sensitivity gallium arsenide phosphide (GaAsP) photomultiplier tube (PMT) detectors. — GaAsP PMTs deliver greater quantum efficiency than standard multialkali PMTs. Fan-less Peltier cooling further improves the signal-to-noise ratio. You can also leverage the advantages of both detector types by combining them in a single system. Image captured with multialkali detector Image captured with GaAsP detector Arc-dVenus...

Spend Less Time Tuning the Laser: Flexible Dual-Line Multiphoton Imaging Multiwavelength Excitation for Multiphoton Imaging The FVMPE-RS imaging platform supports a dual wavelength infrared pulsed laser or two independent infrared lasers for multichannel, multiphoton excitation imaging. You can optimally excite different fluorophores without having to repeatedly tune the laser. Simultaneous excitation with independent power control of each laser line enables users to capture balanced images of different fluorophores. Separate excitation wavelengths for individual fluorophores can also...

Optional Features for Advanced Applications Simultaneous Multiphoton Stimulation and Imaging with the SIM Scanner The SIM scanner, an independent galvanometer scanner, and visible laser modules can be added for precise microsecond photostimulation and photobleaching experiments. On systems with two IR imaging lines, the SIM scanner enables simultaneous multiphoton stimulation and imaging. Synchronize Electrophysiological Data and Laser Light Stimulation with the Analog Unit Analog inputs and TTL I/O are available to support electrophysiology experiments. The analog input unit records...

1. Layout •Select the layout based on your experiment 2. Acquisition Conditions •Reload the settings from previous experiments 3. Acquisition •Acquire multidimensional images •Acquire multiarea images •Acquire images using a complex protocol 4. Viewer •Review images during or after the acquisition using various display methods 5. Analysis •Various image analysis tools are available •Perform analysis via a one-click macro Customizable Layout Customize which controls you see in the software interface and where they are located. Save and reload your favorite layouts. Save the acquisition...

Combine Wide Field of View with High Resolution See your entire specimen at high resolution and in context with the tiling function. This software feature scans multiple adjacent images and stitches them together. With a motorized stage, images can be stitched together in a very wide field of view, while the mapping feature makes it easy to locate the position of specific cells in the larger image. Using the map function with motorized stage, finding target field of view is easy Image data courtesy of: Urs Ziegler and Jose Maria Mateos Center for Microscospy and Image Analysis, University...

Choose From 3 Frames and 3 Laser Configurations Designed for Multiphoton Microscopy Upright Microscope System • Non-descanned detectors are sensitive to ambient light, and the black microscope frame helps reduce undesired light reflections. • A large focus stroke accommodates a range of specimens, from tissue slices to live mice and other small animals. • The optional transmitted fluorescent light detector expands the system’s capabilities in multiphoton imaging. The high NA condenser efficiently collects transmitted fluorescence as well as transmitted second and third harmonic generation...