SPECIFICATION SHEET Human Caldesmon Sandwich ELISA Kit Caldesmon measurement in Human serum samples ■ Sensitivity: Limit of detection (LOD) 2.08 pg/ml. Lowest concentration significantly different from a blank. ■ Assay Range : The assay range [LLOQ - ULOQ] : 25 pg/ml to 800 pg/ml. ULOQ & LLOQ = Highest and Lowest limit of quantification respectively. ■ Precision Intra & Inter-assay : Firalis SAS - www.firalis.com - 35 rue du Fort - F-68330 - Huningue FRANCE +33 389 911 320 Intra-assay Precision : Two samples of known concentration (40 and 700 pg/ml) were tested 30 times within the same plate and by the same operator. Inter-assay Precision : Two samples of known concentration (40 and 700 pg/m) were tested in 10 separate runs. Assays were performed by at least two different operators over two days.

SPECIFICATION SHEET Human Caldesmon Sandwich ELISA Kit Caldesmon measurement in Human serum samples ■ Dilution Linearity : Serum samples containing Caldesmon were serially diluted with assay diluent. Samples exhibits a linear dilution between 2-10. Samples should be diluted for optimal use to at least 1/4 (MRD = 4) ■ Spike Recovery : Recombinant Caldesmon was spiked in serum (n=10) at two concentrations (Low, High) over the range of the assay. ■ Population and clinical data : 100 serum samples from apparently healthy donors (50 female + 50 male) 18-63 years old were evaluated for the presence...