Human Calponin 1 Sandwich ELISA Kit

Product catalog summary

Precision Intra & Inter-assay:
Intra-assay Precision: Two samples with concentrations of 400 and 1500 pg/ml were tested 30 times within the same plate by the same operator.
Inter-assay Precision: The same samples were tested in 10 separate runs by at least three different operators.

Sensitivity:
Limit of detection (LOD) is 14,025 pg/ml, indicating the lowest concentration significantly different from a blank.

Assay Range:
The assay range is from 125 pg/ml to 3000 pg/ml, with ULOQ and LLOQ representing the highest and lowest limits of quantification, respectively.

Precaution:
This kit is for research use only and is not designed for diagnostic or therapeutic procedures.

Spike Recovery:
Recombinant Calponin 1 was spiked in serum at two concentrations over the assay range, showing recovery rates of 97.2% and 95% for samples of 400 and 1500 pg/ml, respectively.

Dilution Linearity:
Serum samples with high concentrations of Calponin 1 were serially diluted, exhibiting linear dilution between 2-30. Samples should be diluted to at least 1/2 (MRD = 2).

Population and Clinical Data:
100 serum samples from healthy donors (50 female, 50 male, aged 18-63) were evaluated. The population mean was 141 +/- 18.72 pg/mL.

Catalog excerpts

SPECIFICATION SHEET Human Calponin 1 Sandwich ELISA Kit Calponin 1 measurement in Human serum samples ■ Sensitivity: Limit of detection (LOD) 14,025 pg/ml. Lowest concentration significantly different from a blank. ■ Assay Range : The assay range [LLOQ - ULOQ] : 125 pg/ml to 3000 pg/ml. ULOQ & LLOQ = Highest and Lowest limit of quantification respectively. ■ Precision Intra & Inter-assay : Firalis SAS - www.firalis.com - 35 rue du Fort - F-68330 - Huningue FRANCE +33 389 911 320 Intra-assay Precision : Two samples of known concentration (400 and 1500 pg/ml) were tested 30 time within the same plate and by the same operator. Inter-assay Precision : Two samples of known concentration (400 and 1500 pg/mi) were tested in 10 separate runs. Assays were performed by at least three different operators.

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( FIRALIS BRIDGING SCIENCE TO CLINICS SPECIFICATION SHEETHuman Calponin 1 Sandwich ELISA Kit Calponin 1 measurement in Human serum samples ■ Dilution Linearity : Serum samples containing high concentrations of Calponin 1 were serially diluted with assay diluent. Samples exhibits a linear dilution between 2-30. Samples should be diluted to at least 1/2 (MRD = 2) ■ Spike Recovery : Recombinant Calponin 1 was spiked in serum (n=10) at two concentrations (Low, High) over the range of the assay. ■ Population and clinical data : 100 serum samples from apparently healthy donors (50 female + 50 male)...

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Human Calponin 1 Sandwich ELISA Kit

Human Calponin 1 Sandwich ELISA Kit

Catalog excerpts

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