SPECIFICATION SHEET Human Calponin 1 Sandwich ELISA Kit Calponin 1 measurement in Human serum samples ■ Sensitivity: Limit of detection (LOD) 14,025 pg/ml. Lowest concentration significantly different from a blank. ■ Assay Range : The assay range [LLOQ - ULOQ] : 125 pg/ml to 3000 pg/ml. ULOQ & LLOQ = Highest and Lowest limit of quantification respectively. ■ Precision Intra & Inter-assay : Firalis SAS - www.firalis.com - 35 rue du Fort - F-68330 - Huningue FRANCE +33 389 911 320 Intra-assay Precision : Two samples of known concentration (400 and 1500 pg/ml) were tested 30 time within the same plate and by the same operator. Inter-assay Precision : Two samples of known concentration (400 and 1500 pg/mi) were tested in 10 separate runs. Assays were performed by at least three different operators.

( FIRALIS BRIDGING SCIENCE TO CLINICS SPECIFICATION SHEETHuman Calponin 1 Sandwich ELISA Kit Calponin 1 measurement in Human serum samples ■ Dilution Linearity : Serum samples containing high concentrations of Calponin 1 were serially diluted with assay diluent. Samples exhibits a linear dilution between 2-30. Samples should be diluted to at least 1/2 (MRD = 2) ■ Spike Recovery : Recombinant Calponin 1 was spiked in serum (n=10) at two concentrations (Low, High) over the range of the assay. ■ Population and clinical data : 100 serum samples from apparently healthy donors (50 female + 50 male)...